Gmür R, McNabb H, van Steenbergen T J, Baehni P, Mombelli A, van Winkelhoff A J, Guggenheim B
Department of Oral Microbiology and General Immunology, Dental Institute, University of Zurich, Switzerland.
Oral Microbiol Immunol. 1993 Apr;8(2):116-20. doi: 10.1111/j.1399-302x.1993.tb00556.x.
Serotyping of 89 Actinobacillus actinomycetemcomitans isolates by indirect immunofluorescence with serotype-specific monoclonal antibodies yielded 10 nontypeable strains. The aim of the present study was to re-evaluate the seroclassification of these strains with a different assay. Electroblotted antigens of nontypeable isolates and selected reference strains were immunostained with 37 human sera containing high antibody titers to A. actinomycetemcomitans. Such immunoblots typically showed an immunodominant, serotype-specific antigen smear. Twelve human sera were found to recognize specifically serotype a, 10 serotype b and 9 serotype c. Three sera contained antibodies to 2 or all 3 serotype antigens; 3 sera had no activity to any reference strain smear antigen. There was complete agreement between the seroclassification of the reference strains with the monoclonal and the polyclonal reagents. Seven of the 10 strains, nontypeable with monoclonal antibodies, could also not be typed by the human sera. Of the remaining 3 strains, one belonged to a previously unrecognized serovar of serotype a, and 2 isolates expressed a novel smear antigen detected by the 3 human sera containing no antibodies to the serotype a, b, or c antigens. We propose that these 2 strains be assigned to a new A. actinomycetemcomitans serotype, designated serotype e.
采用血清型特异性单克隆抗体通过间接免疫荧光法对89株伴放线放线杆菌分离株进行血清分型，结果发现10株无法分型的菌株。本研究的目的是采用不同的检测方法重新评估这些菌株的血清分类。用37份对伴放线放线杆菌具有高抗体滴度的人血清对无法分型的分离株和选定的参考菌株的电印迹抗原进行免疫染色。此类免疫印迹通常显示出一种免疫显性的、血清型特异性的抗原条带。发现12份人血清能特异性识别血清型a，10份识别血清型b，9份识别血清型c。3份血清含有针对2种或所有3种血清型抗原的抗体；3份血清对任何参考菌株的条带抗原均无活性。参考菌株用单克隆和多克隆试剂进行血清分类的结果完全一致。10株用单克隆抗体无法分型的菌株中，有7株也不能用人血清分型。其余3株中，1株属于血清型a先前未识别的血清型，2株分离株表达了一种新的条带抗原，该抗原可被3份不含血清型a、b或c抗原抗体的人血清检测到。我们建议将这2株菌株归为一个新的伴放线放线杆菌血清型，命名为血清型e。
