Pussinen P J, Vilkuna-Rautiainen T, Alfthan G, Mattila K, Asikainen S
Institute of Dentistry, University of Helsinki, Helsinki, Finland.
J Clin Microbiol. 2002 Feb;40(2):512-8. doi: 10.1128/JCM.40.2.512-518.2002.
Periodontitis is a common chronic oral infection caused by gram-negative bacteria, including Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. Periodontitis evokes inflammatory host response locally in the periodontium but also systemically. The systemic humoral antibody response against oral pathogens can conveniently be measured by an immunoassay. The aim of the study was to measure serum immunoglobulin G class antibodies against A. actinomycetemcomitans and P. gingivalis by an enzyme-linked immunosorbent assay (ELISA) in which mixtures of several serotypes of the pathogens were used as antigens to avoid biasing of the results in favor of a particular strain. For A. actinomycetemcomitans the antigen consisted of six strains representing serotypes a, b, c, d, and e and one nonserotypeable strain. In the P. gingivalis ELISA, antigens representing serotypes a, b, and c were used. Serum samples from 90 subjects, including 35 samples from patients with diagnosed periodontitis, 10 samples from periodontally healthy controls, and 45 samples from randomly selected apparently healthy volunteers (referred to as "healthy subjects"), were tested. For both pathogens the antibody levels (means +/- standard deviations) of the patients--xpressed as area under the dilution curve--were significantly higher than those for healthy controls or healthy subjects, with values for A. actinomycetemcomitans and P. gingivalis, respectively, as follows: patients, 22.60 +/- 9.94 mm(2) and 26.72 +/- 11.13 mm(2); healthy controls, 9.99 +/- 3.92 mm(2) and 6.90 +/- 3.38 mm(2); and healthy subjects, 16.85 +/- 6.67 mm(2) and 8.51 +/- 4.23 mm(2). The serotype mixture ELISA is suitable for measuring antibodies against periodontal pathogens in large epidemiological studies in order to evaluate the role of periodontitis as a risk factor for other diseases.
牙周炎是一种由革兰氏阴性菌引起的常见慢性口腔感染，这些细菌包括伴放线放线杆菌和牙龈卟啉单胞菌。牙周炎不仅会在牙周组织局部引发炎症反应，还会引起全身反应。针对口腔病原体的全身体液抗体反应可以通过免疫测定方便地进行检测。本研究的目的是通过酶联免疫吸附测定（ELISA）来检测血清中针对伴放线放线杆菌和牙龈卟啉单胞菌的免疫球蛋白G类抗体，在该测定中使用几种病原体血清型的混合物作为抗原，以避免结果偏向于某一特定菌株。对于伴放线放线杆菌，抗原由代表血清型a、b、c、d和e的六种菌株以及一种无法分型的菌株组成。在牙龈卟啉单胞菌ELISA中，使用了代表血清型a、b和c的抗原。对90名受试者的血清样本进行了检测，其中包括35份来自已确诊牙周炎患者的样本、10份来自牙周健康对照者的样本以及45份从随机选择的表面健康志愿者（称为“健康受试者”）中采集的样本。对于这两种病原体，患者的抗体水平（以稀释曲线下面积表示，均值±标准差）显著高于健康对照者或健康受试者，伴放线放线杆菌和牙龈卟啉单胞菌的抗体水平分别如下：患者，22.60±9.94 mm²和26.72±11.13 mm²；健康对照者，9.99±3.92 mm²和6.90±3.38 mm²；健康受试者，16.85±6.67 mm²和8.51±4.23 mm²。血清型混合物ELISA适用于在大型流行病学研究中检测针对牙周病原体的抗体，以便评估牙周炎作为其他疾病危险因素的作用。
