Mihok S, Olubayo R O, Darji N, Zweygarth E
Tsetse Research Programme, International Centre of Insect Physiology and Ecology, Nairobi, Kenya.
Parasitology. 1993 Jul;107 ( Pt 1):41-8. doi: 10.1017/s0031182000079385.
Trypanosoma congolense, T. brucei and T. simiae isolated from wild-caught Glossina pallidipes were fed to laboratory-reared G. morsitans centralis and G.m. morsitans to determine the effect of host blood at the time of the infective feed on infection rates. Bloodstream forms of trypanosomes were membrane-fed to flies either neat, or mixed with blood from cows, goats, pigs, buffalo, eland, waterbuck and oryx. The use of different bloods for the infective feed resulted in differences in infection rates that were repeatable for both tsetse subspecies and most parasite stocks. Goat, and to a lesser extent, pig blood facilitated infection, producing high infection rates at low parasitaemias. Blood from cows and the wildlife species produced low infection rates, with eland blood producing the lowest. Addition of D(+)-glucosamine (an inhibitor of tsetse midgut lectin) increased infection rates in most cases. These results indicate the presence of species-specific factors in blood that affect trypanosome survival in tsetse. In certain hosts, factors actually appear to promote infection. The nature of these factors and how they might interact with midgut lectins and proteases are discussed.
将从野生捕获的淡足舌蝇（Glossina pallidipes）中分离出的刚果锥虫（Trypanosoma congolense）、布氏锥虫（T. brucei）和西氏锥虫（T. simiae）喂食给实验室饲养的中部 morsitans 采采蝇（G. morsitans centralis）和 morsitans 采采蝇（G.m. morsitans），以确定感染性喂食时宿主血液对感染率的影响。将锥虫的血流形式直接或与来自牛、山羊、猪、水牛、大羚羊、水羚和大羚羊的血液混合后通过膜喂给采采蝇。使用不同的血液进行感染性喂食导致感染率存在差异，这在两种采采蝇亚种和大多数寄生虫种群中都是可重复的。山羊血，以及在较小程度上的猪血，促进了感染，在低寄生虫血症时产生了高感染率。牛血和野生动物的血液产生的感染率较低，其中大羚羊血产生的感染率最低。添加 D(+)-葡萄糖胺（采采蝇中肠凝集素的抑制剂）在大多数情况下提高了感染率。这些结果表明血液中存在影响锥虫在采采蝇中存活的物种特异性因素。在某些宿主中，这些因素实际上似乎促进了感染。讨论了这些因素的性质以及它们可能如何与中肠凝集素和蛋白酶相互作用。
