Maudlin I, Welburn S C
Tsetse Research Laboratory, ODA/University of Bristol, Langford, UK.
Trop Med Parasitol. 1987 Sep;38(3):167-70.
D+Glucosamine, which has been shown in vitro to specifically inhibit tsetse midgut lectin activity, when fed to Glossina morsitans morsitans with the infective feed significantly increased midgut infection rates of Trypanosoma congolense and T. brucei rhodesiense. All flies infected with T.b. rhodesiense and maintained on a diet of blood with D+Glucosamine throughout their lives developed midgut infections. Midgut extracts from flies bred for refractoriness to infection with trypanosomes showed significantly greater erythrocyte agglutinating activity and were more trypanocidal in vitro than flies bred for susceptibility to trypanosome infection. It is concluded that susceptibility to trypanosome infection in tsetse is mediated through midgut lectins.
D + 葡萄糖胺在体外已被证明能特异性抑制采采蝇中肠凝集素活性，当与感染性饲料一起喂给冈比亚采采蝇时，显著提高了刚果锥虫和罗德西亚布氏锥虫的中肠感染率。所有感染罗德西亚布氏锥虫并在其一生中以含D + 葡萄糖胺的血液为食的苍蝇都出现了中肠感染。与易感染锥虫的苍蝇相比，培育出对锥虫感染具有抗性的苍蝇的中肠提取物显示出显著更高的红细胞凝集活性，并且在体外更具杀锥虫作用。得出的结论是，采采蝇对锥虫感染的易感性是通过中肠凝集素介导的。
