Alafiatayo R A, Crawley B, Oppenheim B A, Pentreath V W
Department of Biological Sciences, University of Salford.
Parasitology. 1993 Jul;107 ( Pt 1):49-53. doi: 10.1017/s0031182000079397.
The involvement of endotoxins in Trypanosoma brucei brucei infection in CD-1 mice was investigated by the Limulus amoebocyte lysate (LAL) test. At 7 days post-infection mean serum endotoxin level was elevated by 2.5 times (36.4 pg/ml cf. control 14.25 pg/ml, P < 0.001) and a similar increase was maintained throughout the infection (survival 28-35 days). Purified disrupted parasites contained significant endotoxin activity (mean value 280 pg/mg protein). The mouse infections were also associated with progressive Gram-negative bacteraemia (present in 4 out of 5 infected animals by day 28 p.i.). The increased endotoxin levels may be due to parasite products, the products of intercurrent bacterial infections, other unidentified sources (e.g. from the gut), or a combination of these. It is concluded that the raised endotoxins may be important contributive factors in the pathogenesis of experimental murine trypanosomiasis.
通过鲎试剂试验研究了内毒素在CD - 1小鼠布氏布氏锥虫感染中的作用。感染后7天，血清内毒素平均水平升高了2.5倍（36.4 pg/ml，对照组为14.25 pg/ml，P < 0.001），并且在整个感染过程中（存活28 - 35天）维持了类似的升高。纯化的破碎寄生虫含有显著的内毒素活性（平均值为280 pg/mg蛋白质）。小鼠感染还与进行性革兰氏阴性菌血症相关（感染后28天，5只感染动物中有4只出现菌血症）。内毒素水平升高可能是由于寄生虫产物、并发细菌感染的产物、其他不明来源（如来自肠道）或这些因素的组合。得出的结论是，升高的内毒素可能是实验性鼠锥虫病发病机制中的重要促成因素。
