Davis J R, Beier M S, Beier J C, Pumpuni C B, Edelman R, Herrington D A, Clyde D F
Department of Medicine, University of Maryland School of Medicine, Baltimore.
Am J Trop Med Hyg. 1993 Aug;49(2):174-80. doi: 10.4269/ajtmh.1993.49.174.
We investigated the effects of human anti-sporozoite antibodies on the sporogonic development of Plasmodium falciparum in Anopheles stephensi. Equal volumes of washed human erythrocytes and human sera from 1) volunteers with protective immunity induced by immunization with irradiated P. falciparum sporozoites, 2) the same volunteers before immunization, or 3) Kenyans exposed to natural sporozoite transmission, were fed to cohorts of P. falciparum-infected A. stephensi on either day 5, 8, or 11 after infection. A fourth group of infected mosquitoes from the same cohort were not refed. In two experiments, the effects of anti-sporozoite antibodies were evaluated by determining the infection rates and parasite densities for oocysts and salivary gland sporozoites. There was no evidence that anti-sporozoite antibodies had any effect on the development or intensity of P. falciparum infection in A. stephensi. However, accelerated oocyst maturation was associated with mosquitoes taking a second blood meal, independent of serum source. Salivary gland sporozoites from mosquitoes that fed on immune human sera contained bound human IgG, which was detectable by indirect immunofluorescence assay. The infectivity and transmission potential of human IgG-coated sporozoites is unknown.
我们研究了人抗子孢子抗体对恶性疟原虫在斯氏按蚊体内孢子增殖发育的影响。将等量洗涤后的人红细胞与来自以下人群的人血清混合：1）经辐照的恶性疟原虫孢子免疫诱导产生保护性免疫的志愿者；2）同一志愿者免疫前；或3）接触过自然子孢子传播的肯尼亚人，在感染后第5、8或11天喂给感染恶性疟原虫的斯氏按蚊群体。同一群体的第四组感染蚊子不进行再次喂食。在两项实验中，通过测定卵囊和唾液腺子孢子的感染率和寄生虫密度来评估抗子孢子抗体的作用。没有证据表明抗子孢子抗体对斯氏按蚊体内恶性疟原虫感染的发展或强度有任何影响。然而，卵囊成熟加速与蚊子吸食第二次血餐有关，与血清来源无关。吸食免疫人血清的蚊子唾液腺子孢子含有结合的人IgG，可通过间接免疫荧光测定法检测到。人IgG包被的子孢子的感染性和传播潜力尚不清楚。
