Vaughan J A, Do Rosario V, Leland P, Adjepong A, Light J, Woollett G R, Hollingdale M R, Azad A F
Department of Microbiology & Immunology, University of Maryland School of Medicine, Baltimore 21201.
Exp Parasitol. 1988 Aug;66(2):171-82. doi: 10.1016/0014-4894(88)90088-4.
In endemic areas, malaria-infected mosquitoes may feed upon humans who possess antibodies against malaria sporozoites. Therefore, we examined the effect that ingested anti-sporozoite antibodies have upon Plasmodium falciparum sporogony within Anopheles stephensi mosquitoes. Anti-sporozoite antibodies (IgG) traversed the midgut into the hemocoel within 3 hr following ingestion and, depending upon the titer, persisted for 6-24 hr. When fed to infected A. stephensi at 12 days postinfection (p.i.), anti-sporozoite antibodies bound to sporozoites in the hemocoel, but not to sporozoites residing in the salivary glands of the same mosquitoes. Anti-sporozoite antibodies also bound to developing oocysts when fed to infected A. stephensi at 5 days p.i. Oocysts in mosquitoes that had been fed anti-sporozoite antibodies on Day 5 p.i. produced significantly more sporozoites than did oocysts in nonimmune-fed (Day 5 p.i.) mosquitoes. In addition, the sporozoites from Day 5 immune-fed mosquitoes were significantly more infective to cultured human hepatoma cells than were sporozoites from nonimmune-fed controls. Use of hetereologous immune feedings at Day 5 p.i. did not result in an enhanced production of sporozoites, suggesting that enhancement is related to the specificity of the antibody and is not merely a nutritional effect.
在疟疾流行地区，感染疟原虫的蚊子可能会叮咬体内含有抗疟原虫子孢子抗体的人。因此，我们研究了摄入的抗子孢子抗体对斯氏按蚊体内恶性疟原虫孢子生殖的影响。抗子孢子抗体（IgG）在摄入后3小时内穿过中肠进入血腔，并根据滴度持续存在6 - 24小时。在感染后12天（p.i.）将抗子孢子抗体喂给感染的斯氏按蚊时，抗子孢子抗体在血腔中与子孢子结合，但不与同一蚊子唾液腺中的子孢子结合。在感染后5天（p.i.）将抗子孢子抗体喂给感染的斯氏按蚊时，抗子孢子抗体也与发育中的卵囊结合。在感染后第5天喂食抗子孢子抗体的蚊子中的卵囊产生的子孢子明显多于未免疫喂食（感染后第5天）蚊子中的卵囊。此外，感染后第5天免疫喂食蚊子产生的子孢子对培养的人肝癌细胞的感染性明显高于未免疫喂食对照组产生的子孢子。在感染后第5天使用异种免疫喂食并未导致子孢子产量增加，这表明这种增加与抗体的特异性有关，而不仅仅是营养作用。
