Krajewski W A, Panin V M, Razin S V
Laboratory of Biochemistry, Russian Academy of Sciences, Moscow.
J Biomol Struct Dyn. 1993 Jun;10(6):1013-22. doi: 10.1080/07391102.1993.10508693.
The "rigidity" of chromatin fiber solenoidal structure in different states of condensation was evaluated with the help of gel-electrophoresis. A new property of the unfolded nucleosomal fiber-the capacity to condense with temperature-was demonstrated. These results together with our previously obtained data (W.A. Krajewski et al., Mol. Gen. Genet. 230, pp. 442-448, 1991; W.A. Krajewski et al., Ibid. 231, pp. 17-22, 1991) testify that changes in DNA linking number of transcriptionally active minichromosomes arise in vivo from alteration of nucleosomal solenoid parameters (i.e. from supernucleosomal level of chromatin organization), rather than from core histone modifications only or from increased flexibility of DNA within nucleosomes.
借助凝胶电泳评估了染色质纤维螺线管结构在不同凝聚状态下的“刚性”。证明了展开的核小体纤维的一个新特性——随温度凝聚的能力。这些结果与我们之前获得的数据（W.A.克拉耶夫斯基等人，《分子与普通遗传学》230卷，第442 - 448页，1991年；W.A.克拉耶夫斯基等人，同上，231卷，第17 - 22页，1991年）共同证明，转录活性微型染色体的DNA连接数变化在体内源于核小体螺线管参数的改变（即染色质组织的超核小体水平），而非仅源于核心组蛋白修饰或核小体内DNA柔韧性的增加。
