Saito A, Kimura J, Shionoya K, Kuriyama T
Resources & Environment Protection Research Laboratories, NEC Corporation, Kanagawa, Japan.
Biosens Bioelectron. 1993;8(3-4):149-54. doi: 10.1016/0956-5663(93)85026-k.
An ion-sensitive field-effect transistor (ISFET) glucose sensor with a new membrane structure is fabricated and its characteristics are evaluated in continuous measurement of non-diluted serum glucose. This sensor chip is composed of two ISFETs and a gold electrode. An albumin-glutaraldehyde crosslinked membrane, patterned on one of the ISFET gates and the gold electrode, contacts an enzyme membrane deposited on the other gate in edge to edge. Making an electrical connection between the gold electrode and two ISFETs, this membrane enables stable sensor responses to be made. This sensor is mounted on a flow cell into which serum samples and air are alternately fed. The sensor output shows a good linear relationship, up to 300 mg dl-1 glucose, and correlates well with a conventional glucose analyzer. This sensor shows promise as a key device in the development of portable glucose monitors.
制备了一种具有新型膜结构的离子敏感场效应晶体管（ISFET）葡萄糖传感器，并在连续测量未稀释血清葡萄糖的过程中对其特性进行了评估。该传感器芯片由两个ISFET和一个金电极组成。在其中一个ISFET栅极和金电极上形成图案的白蛋白-戊二醛交联膜与沉积在另一个栅极上的酶膜边缘对边缘接触。通过在金电极和两个ISFET之间建立电连接，这种膜能够实现稳定的传感器响应。该传感器安装在一个流通池中，血清样品和空气交替注入其中。传感器输出在高达300 mg dl-1葡萄糖的范围内显示出良好的线性关系，并且与传统葡萄糖分析仪具有良好的相关性。该传感器有望成为便携式葡萄糖监测仪开发中的关键器件。
