Håkanson H, Kyröläinen M, Mattiasson B
Department of Biotechnology, Chemical Centre, Lund, Sweden.
Biosens Bioelectron. 1993;8(3-4):213-7. doi: 10.1016/0956-5663(93)85036-n.
The lactate profile in serum was monitored continuously in volunteers under physical exercise on a test bicycle. The assay was operated ex vivo by means of continuous sampling through a coaxial catheter. After dialysis online, lactate was converted by means of lactate oxidase immobilized to porous glass, and the depletion of oxygen was registered by means of a Clark electrode. Good agreement between the continuous monitoring and off-line analyses was found.
在测试自行车上对志愿者进行体育锻炼时,持续监测其血清中的乳酸水平。该测定通过同轴导管连续采样在体外进行。在线透析后,乳酸通过固定在多孔玻璃上的乳酸氧化酶进行转化,氧气的消耗通过克拉克电极进行记录。发现连续监测与离线分析之间具有良好的一致性。