Fujino K, Kitahashi S, Nakao M, Shimojo N, Okuda K
Central Clinical Laboratory, Osaka City University Medical School.
Rinsho Byori. 1992 Sep;40(9):982-6.
The anaerobic threshold is useful for estimating the intensity of physical exercise. It is shown as either an increase in blood concentrations of lactate or a disproportionate increase in ventilation. We developed a lactate analyzer based on an electroenzymatic method with a continuous blood sampling system through a double-lumen catheter. Ascorbic acid, bilirubin, hemoglobin, creatinine, uric acid, and glucose did not interfere the results. The lactate concentrations in blood samples from healthy subjects during physical exercise correlated well (r = 0.993) with results measured by the conventional enzymatic method. We measured the concentrations of blood lactate with a use of this lactate analyzer to see the anaerobic threshold in nine healthy volunteers during exercise on a treadmill with an increasing workload. The point at which lactate concentrations started to increase was detected easily. The anaerobic threshold identified as a disproportionate increase in ventilation was seen at almost the same time. We conclude that the lactate analyzer, with a continuous blood sampling system, can measure precisely concentrations of lactate in blood and can detect the anaerobic threshold during physical exercise.
无氧阈对于估计体育锻炼的强度很有用。它表现为血液中乳酸浓度的增加或通气量不成比例的增加。我们基于一种酶促电分析方法开发了一种乳酸分析仪，并通过双腔导管建立了连续血液采样系统。抗坏血酸、胆红素、血红蛋白、肌酐、尿酸和葡萄糖均不干扰检测结果。健康受试者在体育锻炼期间血样中的乳酸浓度与传统酶法测得的结果具有良好的相关性（r = 0.993）。我们使用这种乳酸分析仪测量了九名健康志愿者在跑步机上进行工作量递增运动时的血乳酸浓度，以观察无氧阈。乳酸浓度开始增加的点很容易被检测到。几乎与此同时，无氧阈表现为通气量不成比例的增加。我们得出结论，配备连续血液采样系统的乳酸分析仪能够精确测量血液中的乳酸浓度，并能检测体育锻炼期间的无氧阈。
