Yoshiike T, Aikawa Y, Sindhvananda J, Suto H, Nishimura K, Kawamoto T, Ogawa H
Department of Dermatology, Juntendo University School of Medicine, Tokyo, Japan.
J Dermatol Sci. 1993 Apr;5(2):92-6. doi: 10.1016/0923-1811(93)90076-2.
The existence of a defect in the skin barrier of patients with atopic dermatitis (AD) was demonstrated and its importance in the pathogenesis of AD was emphasized. In order to evaluate the penetration properties of the stratum corneum of AD patients, the in vivo skin response to the penetration of dimethyl sulfoxide (DMSO) and in vitro response to the penetration of theophylline utilizing a diffusion chamber were studied. Both methods demonstrated an increasing level of penetration through the epidermal stratum corneum, with greatest penetration being evident with lesional skin, followed by AD non-lesional and then the normal control. However, statistical significances existed only between non-lesional and lesional skins in the case of the DMSO test, and between the normal control and non-lesional skin in the case of the diffusion chamber analysis using theophylline. Increased penetration of a non-specific nature is important in the pathogenesis of AD.
特应性皮炎（AD）患者皮肤屏障缺陷的存在得到了证实，并强调了其在AD发病机制中的重要性。为了评估AD患者角质层的渗透特性，研究了二甲基亚砜（DMSO）渗透的体内皮肤反应以及利用扩散室对茶碱渗透的体外反应。两种方法均显示穿过表皮角质层的渗透水平增加，病变皮肤的渗透最为明显，其次是AD非病变皮肤，然后是正常对照。然而，在DMSO试验中，仅非病变皮肤与病变皮肤之间存在统计学差异；在使用茶碱的扩散室分析中，仅正常对照与非病变皮肤之间存在统计学差异。非特异性的渗透增加在AD发病机制中很重要。
