Wiedemann G, Jonetz-Mentzel L, Panse R
Klinisch-Chemisches Labor, Klinik und Poliklinik für Kindermedizin der Medizinischen Hochschule, Erfurt, Germany.
Eur J Clin Chem Clin Biochem. 1993 May;31(5):277-88. doi: 10.1515/cclm.1993.31.5.277.
Thyrotropin, triiodothyronine, thyroxine and free thyroxine were determined in the sera of 714 euthyreotic neonates, infants, children and adolescents (age range 5 days to 18 years), using the IMx from Abbott Laboratories. Thyrotropin, triiodothyronine and free thyroxine were determined with microparticle enzyme immunoassays (MEIA). Thyroxine was determined with a fluorescence polarization immunoassay (FPIA). The proband collective was divided into 9 age groups, and each age group into males and females. In accordance with the recommendations of the International Federation of Clinical Chemistry, the 95% scatter range was taken as the reference range. In the different age groups, the ranges of some hormones showed significant differences between males and females. When no significant difference existed between the sexes, the results for males and females were evaluated statistically as a single group. Only a few reference groups showed normal Gaussian distributions. Therefore, in addition to the 50th percentile, the 2.5th and 97.5th percentiles were also calculated for all reference groups. Minimal and maximal values were also determined. The U-test of Mann & Whitney was used to test for significant differences between individual reference groups, and groups showing no significant differences were combined. The corresponding references ranges were then calculated.
使用雅培实验室的IMx测定了714名甲状腺功能正常的新生儿、婴儿、儿童和青少年（年龄范围为5天至18岁）血清中的促甲状腺激素、三碘甲状腺原氨酸、甲状腺素和游离甲状腺素。促甲状腺激素、三碘甲状腺原氨酸和游离甲状腺素采用微粒体酶免疫测定法（MEIA）测定。甲状腺素采用荧光偏振免疫测定法（FPIA）测定。将研究对象群体分为9个年龄组，每个年龄组再分为男性和女性。根据国际临床化学联合会的建议，将95%的离散范围作为参考范围。在不同年龄组中，某些激素的范围在男性和女性之间存在显著差异。当两性之间不存在显著差异时，将男性和女性的结果作为一个单一群体进行统计学评估。只有少数参考组呈现正态高斯分布。因此，除了计算第50百分位数外，还为所有参考组计算了第2.5和第97.5百分位数。还确定了最小值和最大值。使用曼恩和惠特尼U检验来检验各个参考组之间的显著差异，并将无显著差异的组合并。然后计算相应的参考范围。
