Weiss D, van der Luit A H, Kroon J T, Mol J N, Kooter J M
Department of Genetics, Vrije Universiteit, Amsterdam, Netherlands.
Plant Mol Biol. 1993 Aug;22(5):893-7. doi: 10.1007/BF00027374.
The synthesis of anthocyanins in higher plants involves many enzymatic steps. Here we describe the isolation and characterization of a cDNA, ant17, which encodes a protein that has 73% amino acid sequence identity with the candi gene product of Antirrhinum majus and 48% with that of the maize a2 gene. This protein may therefore be involved in the synthesis of anthocyanins in the steps after the action of dihydroflavonol 4-reductase. This is consistent with the absence of ant17 expression in the regulatory anthocyanin mutants of petunia an1, an2 and an11. Furthermore, ant17 is predominantly expressed in corollas and anthers and is induced by gibberellic acid.
高等植物中花青素的合成涉及许多酶促步骤。在此，我们描述了一个cDNA（ant17）的分离和特性，该cDNA编码的蛋白质与金鱼草的candi基因产物具有73%的氨基酸序列同一性，与玉米a2基因产物具有48%的同一性。因此，该蛋白质可能在二氢黄酮醇4-还原酶作用后的步骤中参与花青素的合成。这与矮牵牛an1、an2和an11的调节性花青素突变体中ant17不表达是一致的。此外，ant17主要在花冠和花药中表达，并受赤霉素诱导。
