Neuteboom S T, Hulleman E, Schilperoort R A, Hoge J H
Institute of Molecular Plant Sciences, Leiden University, Clusius Laboratory, Netherlands.
Plant Mol Biol. 1993 Aug;22(5):923-9. doi: 10.1007/BF00027379.
The promoter region of the Agrobacterium tumefaciens T-cyt gene was fused to a beta-glucuronidase (gusA) reporter gene and introduced into tobacco plants. Detection of gusA expression in transgenic F1 progeny revealed that the T-cyt promoter is active in many, if not all, cell types in leaves, stems and roots of fully developed plants. Developmental stage-dependent promoter activity was observed in seedlings. Analysis of 5'-deleted promoter fragments showed that sequences located between positions -185 and -139 with respect to the T-cyt translational start codon are essential for T-cyt promoter activity in transfected tobacco protoplasts as well as in transformed tobacco plants.
将根癌农杆菌T-cyt基因的启动子区域与β-葡萄糖醛酸酶（gusA）报告基因融合，并导入烟草植株。对转基因F1后代中gusA表达的检测表明，T-cyt启动子在完全发育植株的叶、茎和根的许多（如果不是所有）细胞类型中具有活性。在幼苗中观察到了发育阶段依赖性的启动子活性。对5'-缺失启动子片段的分析表明，相对于T-cyt翻译起始密码子，位于-185至-139位置之间的序列对于转染烟草原生质体以及转化烟草植株中的T-cyt启动子活性至关重要。
