Bonhomme A, Pingret L, Bonhomme P, Michel J, Balossier G, Lhotel M, Pluot M, Pinon J M
Unité INSERM U.314, Hôpital Maison Blanche, Reims, France.
Microsc Res Tech. 1993 Jul 1;25(4):276-85. doi: 10.1002/jemt.1070250403.
The localization of calcium in Toxoplasma gondii tachyzoites was studied at the ultrastructural level, with a cytochemical pyroantimonate precipitation method (PA) and controlled by EGTA chelating and EDX and EELS microanalyses. Appropriate conditions for material preparation, fixation and embedding, were defined. The proportion of precipitates that were either free or inside vacuoles and their distribution inside Toxoplasma appeared to be PA dose-dependent. Precipitation mainly occurred in the anterior pole of the Epon-embedded tachyzoites. EDX and EELS analyses showed that out of 30 PA precipitates inside tachyzoites, 78% contained Ca. In Melamine sections, 96% of the tachyzoites had intracellular precipitates and the membrane complex was stained; 25% of the tachyzoites inside host cells contained PA-Ca precipitates, but most of them were retained in the reticular network of the parasitophorous vacuole. Melamine embedding appeared to improve the preservation of calcium pyroantimonate precipitates.
采用细胞化学焦锑酸盐沉淀法（PA）在超微结构水平研究了钙在刚地弓形虫速殖子中的定位，并通过EGTA螯合以及EDX和EELS微量分析进行对照。确定了材料制备、固定和包埋的合适条件。游离或存在于液泡内的沉淀物比例及其在弓形虫内的分布似乎与PA剂量有关。沉淀主要发生在环氧树脂包埋的速殖子的前端。EDX和EELS分析表明，在速殖子内的30个PA沉淀物中，78%含有钙。在三聚氰胺切片中，96%的速殖子有细胞内沉淀且膜复合体被染色；宿主细胞内25%的速殖子含有PA-Ca沉淀，但其中大多数保留在寄生泡的网状网络中。三聚氰胺包埋似乎改善了焦锑酸钙沉淀的保存。
