Nonomura A, Mizukami Y, Oda M, Shimizu J, Watanabe Y, Kamimura R, Takashima T
Pathology Section, Kanazawa University Hospital, School of Medicine, Kanazawa University, Japan.
Surg Today. 1993;23(6):486-90. doi: 10.1007/BF00730621.
Nucleolar organizer regions (NORs) were investigated in lung carcinomas by silver staining. This method was applied to 111 lung carcinoma specimens, including 40 with squamous cell carcinoma (SCC), 42 with adenocarcinoma (ADENO), 8 with adenosquamous carcinoma (ADESQ), 8 with small cell carcinoma (SMCC), 6 with large cell carcinoma (LGCC), and 7 with typical carcinoid tumors (CAOID). The mean AgNOR counts of ADENO, SCC, ADESQ, SMCC, and LGCC were significantly higher than those of the normal bronchial surface and those of the glandular or alveolar epithelium. The mean AgNOR count of CAOID was significantly higher than those of the normal glandular and alveolar epithelium but not that of the surface epithelium. The mean AgNOR count of SCC was significantly higher than that of bronchial squamous metaplasia, and the count of SMCC was significantly higher than that of CAOID. Within the same cancer category, the mean number of AgNORs increased in parallel with the histological tumor grades. These results indicate that the AgNOR method is useful for differentiating lung carcinoma from its normal counterparts and for evaluating histological tumor grades in the same lineage of lung carcinoma.
采用银染色法对肺癌中的核仁组织区（NORs）进行研究。该方法应用于111例肺癌标本，其中包括40例鳞状细胞癌（SCC）、42例腺癌（ADENO）、8例腺鳞癌（ADESQ）、8例小细胞癌（SMCC）、6例大细胞癌（LGCC）和7例典型类癌（CAOID）。腺癌、鳞状细胞癌、腺鳞癌、小细胞癌和大细胞癌的平均银染核仁组成区计数显著高于正常支气管表面以及腺上皮或肺泡上皮的计数。典型类癌的平均银染核仁组成区计数显著高于正常腺上皮和肺泡上皮，但不高于表面上皮。鳞状细胞癌的平均银染核仁组成区计数显著高于支气管鳞状化生，小细胞癌的计数显著高于典型类癌。在同一癌症类别中，银染核仁组成区的平均数与组织学肿瘤分级平行增加。这些结果表明，银染核仁组成区方法有助于将肺癌与其正常对应物区分开来，并用于评估同一谱系肺癌中的组织学肿瘤分级。
