Abe S, Sukoh N, Ogura S, Kunikane H, Watanabe N, Nakajima I, Takekawa H, Kawakami Y
Third Department of Medicine, Sapporo Medical College.
Thorax. 1992 Oct;47(10):778-80. doi: 10.1136/thx.47.10.778.
The numbers of nucleolar organiser regions (AgNORs) per cell has been considered as an indicator of the cellular proliferative activity. A study was carried out to examine whether AgNOR numbers relate to the growth rate in squamous cell carcinoma of the lung.
AgNORs were stained by a one step silver method, and examined in representative paraffin sections from 45 cases of squamous cell carcinoma of the lung treated by surgical resection of the primary tumour.
The mean (SD) AgNOR numbers per cell in squamous cell carcinomas (5.3 (0.9)) were significantly higher than those in normal bronchial epithelium (1.2 (0.1)). There was no statistical difference among tumours of different post-surgical stages (stage I = 5.2 (0.8), II = 5.9 (1.4), III A = 5.5 (1.3)). The tumour volume doubling time in these cases ranged from 74 to 208 days (120.7 (40.4)). There was a high inverse correlation between the AgNOR numbers and doubling time.
The AgNOR numbers were related to the growth rate of squamous cell carcinoma of the lung. Thus the AgNOR count could be used as a useful marker for investigating the cellular proliferative activity.
细胞内核仁组成区（AgNORs）的数量被视为细胞增殖活性的指标。开展了一项研究以检验AgNOR数量是否与肺鳞状细胞癌的生长速率相关。
采用一步银染法对AgNORs进行染色，并在45例经手术切除原发肿瘤的肺鳞状细胞癌的代表性石蜡切片中进行检测。
肺鳞状细胞癌中每个细胞的平均（标准差）AgNOR数量（5.3（0.9））显著高于正常支气管上皮中的数量（1.2（0.1））。不同术后分期的肿瘤之间无统计学差异（I期 = 5.2（0.8），II期 = 5.9（1.4），IIIA期 = 5.5（1.3））。这些病例的肿瘤体积倍增时间为74至208天（120.7（40.4））。AgNOR数量与倍增时间呈高度负相关。
AgNOR数量与肺鳞状细胞癌的生长速率相关。因此，AgNOR计数可作为研究细胞增殖活性的有用标志物。
