具有集成辅因子再生的化学酶促半乳糖唾液酸化
Chemoenzymatic galactosialylation with integrated cofactor regeneration.
作者信息
Stangier P, Treder W, Thiem J
机构信息
Institut für Organische Chemie, Universität Hamburg, Germany.
出版信息
Glycoconj J. 1993 Feb;10(1):26-33. doi: 10.1007/BF00731183.
PMID:8358224
Abstract
As a precursor for the chemical synthesis of sialylated oligosaccharides, the trisaccharide glycoside Neu5Ac alpha (2-8)Gal beta (1-4)GlcNAc beta (1-O)-pent-4-ene was synthesized starting from GlcNAc beta (1-O)-pent-4-ene, UDP-glucose and N-acetylneuraminic acid in a one pot reaction employing galactosyltransferase and alpha (2-6)sialyltransferase in a complete cofactor regeneration system.
摘要
作为唾液酸化寡糖化学合成的前体，三糖糖苷Neu5Acα(2 - 8)Galβ(1 - 4)GlcNAcβ(1 - O)-戊 - 4 - 烯是从GlcNAcβ(1 - O)-戊 - 4 - 烯、UDP - 葡萄糖和N - 乙酰神经氨酸出发，在一个完整的辅因子再生系统中，通过一锅法反应，利用半乳糖基转移酶和α(2 - 6)唾液酸转移酶合成的。
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