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一年生鱼类胚胎的蛋白水解脱膜

Proteolytic dechorionation of annual fish embryos.

作者信息

Lesseps R J, Gast E A

出版信息

Anat Rec. 1977 Jan;187(1):125-8. doi: 10.1002/ar.1091870110.

DOI:10.1002/ar.1091870110
PMID:835841
Abstract

Incubation of early embryos of Nothobranchius korthausae in sterile Tris-buffered pronase with added salts and EDTA results in complete dechorionation, after which development proceeds normally and at the same rate as in control embryos. The time required for dechorionation varies according to the age of the embryo and the concentration of pronase employed.

摘要

将科氏假鳃鳉的早期胚胎置于添加了盐类和乙二胺四乙酸(EDTA)的无菌Tris缓冲链霉蛋白酶中进行孵育,胚胎会完全去膜,之后发育正常,且发育速度与对照胚胎相同。去膜所需时间根据胚胎的年龄和所用链霉蛋白酶的浓度而有所不同。

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引用本文的文献

1
The stage-dependent resistance of the chorion to external chemical damage and its relationship to embryonic diapause in the annual fish, Nothobranchius guentheri.一年生鱼类冈氏假鳃鳉(Nothobranchius guentheri)中绒毛膜对外部化学损伤的阶段依赖性抗性及其与胚胎滞育的关系。
Experientia. 1984 Jul 15;40(7):753-4. doi: 10.1007/BF01949761.
2
Cytosolic free calcium-ion concentration in cleaving embryonic cells of Oryzias latipes measured with calcium-selective microelectrodes.用钙选择性微电极测量青鳉胚胎分裂细胞中的胞质游离钙离子浓度。
J Cell Biol. 1985 Mar;100(3):947-54. doi: 10.1083/jcb.100.3.947.