Lesseps R J, Gast E A
Anat Rec. 1977 Jan;187(1):125-8. doi: 10.1002/ar.1091870110.
Incubation of early embryos of Nothobranchius korthausae in sterile Tris-buffered pronase with added salts and EDTA results in complete dechorionation, after which development proceeds normally and at the same rate as in control embryos. The time required for dechorionation varies according to the age of the embryo and the concentration of pronase employed.
将科氏假鳃鳉的早期胚胎置于添加了盐类和乙二胺四乙酸(EDTA)的无菌Tris缓冲链霉蛋白酶中进行孵育,胚胎会完全去膜,之后发育正常,且发育速度与对照胚胎相同。去膜所需时间根据胚胎的年龄和所用链霉蛋白酶的浓度而有所不同。