3400个新的表达序列标签揭示了人类大脑中转录本的多样性。

3,400 new expressed sequence tags identify diversity of transcripts in human brain.

作者信息

Adams M D, Kerlavage A R, Fields C, Venter J C

机构信息

Receptor Biochemistry and Molecular Biology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892.

出版信息

Nat Genet. 1993 Jul;4(3):256-67. doi: 10.1038/ng0793-256.

DOI:10.1038/ng0793-256

PMID:8358434

Abstract

We present the results of the partial sequencing of over 3,400 expressed sequence tags (ESTs) from human brain cDNA clones, which increases the number of distinct genes expressed in the brain, that are represented by ESTs, to about 6,000. By choosing clones in an unbiased manner, it is possible to construct a profile of the transcriptional activity of the brain at different stages. Proteins that comprise the cytoskeleton are the most abundant; however, a large variety of regulatory proteins are also seen. About half of the ESTs predicted to contain a protein-coding region have no matches in the public peptide databases and may represent new gene families.

摘要

我们展示了对来自人脑cDNA克隆的3400多个表达序列标签（EST）进行部分测序的结果，这使得以EST表示的在脑中表达的不同基因数量增加到约6000个。通过以无偏差的方式选择克隆，有可能构建不同阶段脑转录活性的概况。构成细胞骨架的蛋白质最为丰富；然而，也发现了各种各样的调节蛋白。预计含有蛋白质编码区的EST中约有一半在公共肽数据库中没有匹配项，可能代表新的基因家族。