高胆固醇血症严重损害兔离体耳急性动脉闭塞后依赖内皮舒张因子的侧支循环灌注。

Hypercholesterolaemia severely impairs EDRF-dependent collateral perfusion following acute arterial occlusion in rabbit isolated ear.

作者信息

Randall M D, Smith J A, Griffith T M

机构信息

Department of Diagnostic Radiology, University of Wales College of Medicine, Health Park, Cardiff.

出版信息

Br J Pharmacol. 1993 Jul;109(3):838-44. doi: 10.1111/j.1476-5381.1993.tb13651.x.

DOI:10.1111/j.1476-5381.1993.tb13651.x

PMID:8358574

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2175643/

Abstract

We have used a rabbit isolated buffer-perfused ear as a model of acute arterial occlusion to investigate the effects of dietary-induced hypercholesterolaemia on EDRF-dependent collateral perfusion. The effects of hypercholesterolaemia on endothelium-dependent relaxations to acetylcholine were also investigated in the unligated isolated buffer-perfused ear of the rabbit. 2. In rabbits receiving a high cholesterol diet (1%) for 4 weeks, blood cholesterol levels were significantly (P < 0.001) increased (26.0 +/- 3.6 vs. 2.6 +/- 0.6 mmol l-1), EDRF-dependent collateral perfusion was similar to that in age-matched controls for the first 15 min after occlusion but then decreased and was significantly (P < 0.01) less than control during the maintenance phase of collateral perfusion. 3. Cholesterol feeding for 8 weeks (blood cholesterol = 56.2 +/- 3.8 vs. 1.3 +/- 0.1 mmol l-1) was associated with almost complete impairment of collateral perfusion, an effect previously observed following inhibition of EDRF synthesis. 4. Endothelium-dependent relaxations to acetylcholine in isolated perfused ears were impaired in the rabbits fed the diet for 8 weeks but not those fed for 4 weeks. In the 8 week group, the maximum relaxation of tone was 32.6 +/- 11.6% and was significantly (P < 0.01) less than that in the controls (77.9 +/- 5.7%). 5. We conclude that EDRF-dependent collateral perfusion is severely impaired in hypercholesterolaemia and that the level of impairment is related to the duration of feeding.

摘要

我们使用兔离体缓冲液灌注耳作为急性动脉闭塞模型，以研究饮食诱导的高胆固醇血症对内皮舒张因子（EDRF）依赖性侧支循环灌注的影响。还在未结扎的兔离体缓冲液灌注耳中研究了高胆固醇血症对乙酰胆碱诱导的内皮依赖性舒张的影响。2. 给兔喂食高胆固醇饮食（1%）4周后，血液胆固醇水平显著升高（P < 0.001）（26.0±3.6 vs. 2.6±0.6 mmol l-1），EDRF依赖性侧支循环灌注在闭塞后的前15分钟与年龄匹配的对照组相似，但随后下降，在侧支循环灌注维持阶段显著低于对照组（P < 0.01）。3. 喂食胆固醇8周（血液胆固醇 = 56.2±3.8 vs. 1.3±0.1 mmol l-1）与侧支循环灌注几乎完全受损有关，这一效应先前在抑制EDRF合成后也有观察到。4. 喂食8周的兔离体灌注耳中对乙酰胆碱的内皮依赖性舒张受损，但喂食4周的兔未出现这种情况。在8周组中，最大舒张张力为32.6±11.6%，显著低于对照组（77.9±5.7%）（P < 0.01）。5. 我们得出结论，高胆固醇血症中EDRF依赖性侧支循环灌注严重受损，且受损程度与喂食持续时间有关。