一种用于直接检测临床标本中腺病毒的酶免疫测定法。

An enzyme immunoassay for the direct detection of adenovirus in clinical specimens.

作者信息

Gleaves C A, Militoni J, Ashley R L

机构信息

Diagnostic Virology Laboratory, Fred Hutchinson Cancer Research Center, Seattle, Washington.

出版信息

Diagn Microbiol Infect Dis. 1993 Jul;17(1):57-9. doi: 10.1016/0732-8893(93)90071-e.

DOI:10.1016/0732-8893(93)90071-e

PMID:8359007

Abstract

A total of 414 clinical samples were tested by an enzyme immunoassay (EIA) for direct detection of adenovirus as well as cultured in Graham 293, A549, and human diploid foreskin fibroblast cells. Adenovirus was detected in 69 (16.7%) of 414 clinical specimens. The EIA detected adenovirus in 51 (12.3%) of 414 specimens; two were EIA positive only. Adenovirus was isolated from 67 (16.2%) of 414 specimens; 18 were culture positive only. Compared with viral isolation, EIA had a sensitivity of 73.4% and a specificity of 99.4%, with a positive predictive value of 96% and a negative predictive value of 95%.

摘要

共有414份临床样本通过酶免疫测定法（EIA）进行检测，以直接检测腺病毒，并在格雷厄姆293细胞、A549细胞和人二倍体包皮成纤维细胞中培养。在414份临床标本中，有69份（16.7%）检测到腺病毒。EIA在414份标本中的51份（12.3%）检测到腺病毒；两份仅EIA呈阳性。从414份标本中的67份（16.2%）分离出腺病毒；18份仅培养呈阳性。与病毒分离相比，EIA的敏感性为73.4%，特异性为99.4%，阳性预测值为96%，阴性预测值为95%。