用于检测腺病毒抗原的一种商用单克隆抗体的评估
Evaluation of a commercial monoclonal antibody for detection of adenovirus antigen.
作者信息
August M J, Warford A L
机构信息
SmithKline Bio-Science Laboratories, Van Nuys, California 91405.
出版信息
J Clin Microbiol. 1987 Nov;25(11):2233-5. doi: 10.1128/jcm.25.11.2233-2235.1987.
Abstract
The Adenoclone monoclonal antibody for detection of adenovirus antigen was evaluated by enzyme immunoassay and a 72-h indirect fluorescent-antibody test in shell vials and compared with standard culture. The sensitivity and specificity of the Adenoclone test were both 100% with 114 cell culture isolates. With 310 direct clinical specimens, the Adenoclone enzyme immunoassay sensitivity varied from 14.3 to 66.6%, but the Adenoclone indirect fluorescent-antibody test sensitivity was 86.7%, with 100% specificity.
摘要
采用酶免疫测定法及在细胞培养管中进行的72小时间接荧光抗体试验，对用于检测腺病毒抗原的Adenoclone单克隆抗体进行了评估，并与标准培养法进行比较。对于114株细胞培养分离株，Adenoclone试验的敏感性和特异性均为100%。对于310份直接临床标本，Adenoclone酶免疫测定法的敏感性在14.3%至66.6%之间变化，但Adenoclone间接荧光抗体试验的敏感性为86.7%，特异性为100%。
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