Mailhes J B, Aardema M J, Marchetti F
Department of Obstetrics and Gynecology, Louisiana State University Medical Center, Shreveport 71130.
Environ Mol Mutagen. 1993;22(2):107-14. doi: 10.1002/em.2850220209.
The various causative and mechanistic phenomena associated with aneuploidy induction require considerable investigation to better understand the etiology of chromosome missegregation. We investigated the potential of vinblastine sulfate, pyrimethamine, diethylstilbestrol diphosphate, and chloral hydrate to induce numerical and structural chromosome changes in female mouse germ cells. Superovulated ICR mice were administered the compounds either by intraperitoneal injection or oral gavage, and oocytes were collected and processed for cytogenetic analysis 17 hr later. Vinblastine sulfate, administered i.p., induced a significant increase in the frequency of ovulated MI oocytes and of hyperploid MII oocytes compared to controls, but did not increase the frequency of structural aberrations. Pyrimethamine, diethylstilbestrol diphosphate, and chloral hydrate did not increase the frequency of numerical or structural chromosome changes in female mouse germ cells.
与非整倍体诱导相关的各种致病和机制现象需要大量研究，以更好地理解染色体错分离的病因。我们研究了硫酸长春碱、乙胺嘧啶、己烯雌酚二磷酸酯和水合氯醛在雌性小鼠生殖细胞中诱导染色体数目和结构变化的潜力。对超排卵的ICR小鼠通过腹腔注射或灌胃给予这些化合物，17小时后收集卵母细胞并进行细胞遗传学分析。与对照组相比，腹腔注射硫酸长春碱可使排卵的MI期卵母细胞和超倍体MII期卵母细胞的频率显著增加，但并未增加结构畸变的频率。乙胺嘧啶、己烯雌酚二磷酸酯和水合氯醛并未增加雌性小鼠生殖细胞中染色体数目或结构变化的频率。
