Acquaye C, Johnson R M
Department of Biochemistry, Wayne State University Medical School, Detroit, MI.
Exp Hematol. 1993 Sep;21(10):1358-60.
Cellulose columns have been used to separate erythrocytes into deformability classes, but recoveries have been variable and incomplete. Columns of modified cellulose (propylaminocarbonylmethyl cellulose [PAC] and butylisourea cellulose [BIC]) were effective in increasing the recovery of both normal and sickle cells applied to the columns, with reasonable yields of rigid cells in the late fractions. In particular, sickle cells were recovered in 95% yield, and late eluting cells had a sharply reduced deformability index.
纤维素柱已被用于将红细胞分离成不同的可变形性类别，但回收率各不相同且不完整。改性纤维素柱（丙胺羰基甲基纤维素[PAC]和丁基异脲纤维素[BIC]）能有效提高施加到柱上的正常细胞和镰状细胞的回收率，后期馏分中刚性细胞的产量合理。特别是，镰状细胞的回收率达到了95%，且后期洗脱的细胞的可变形性指数急剧降低。
