Vyas J J, Redkar A A, Kabre S S, Mittra I
Department of Surgery, Tata Memorial Hospital, Bombay, India.
Eur J Surg Oncol. 1993 Aug;19(4):368-71.
To investigate the possibility of receptor degradation due to devascularization of the tumour during mastectomy, oestrogen and progesterone receptors (ER and PgR) were measured by an enzyme-immunoassay (EIA) in 59 cases of primary breast cancer on samples taken before and after performing a modified radical mastectomy. Pre- and post-mastectomy samples from the same patient were analysed simultaneously in the same assay run. There was 86.4% and 93.2% agreement respectively in ER and PgR status between samples removed before and after surgery. When actual values were analysed, the post-mastectomy values were higher or lower than pre-mastectomy values with similar frequency. These random variations could be attributed to heterogeneous distribution of receptors within a tumour. The overall correlation between pre- and post-mastectomy values was excellent (ER: r = 0.810, P < 0.001; PgR: r = 0.706, P < 0.001). Devascularization of the tumour during surgery does not seem to affect the integrity of the epitopes recognized by monoclonal antibodies against ER and PgR to any significant extent.
为研究在乳房切除术期间肿瘤因血管化缺失而导致受体降解的可能性，采用酶免疫测定法（EIA）对59例原发性乳腺癌患者在改良根治性乳房切除术前、后的样本进行雌激素和孕激素受体（ER和PgR）检测。同一患者乳房切除术前、后的样本在同一次检测中同时进行分析。手术前、后切除样本的ER和PgR状态一致性分别为86.4%和93.2%。分析实际值时，乳房切除术后的值高于或低于乳房切除术前的值的频率相近。这些随机变化可能归因于肿瘤内受体的异质性分布。乳房切除术前、后值之间的总体相关性良好（ER：r = 0.810，P < 0.001；PgR：r = 0.706，P < 0.001）。手术期间肿瘤的血管化缺失似乎并未在任何显著程度上影响抗ER和PgR单克隆抗体所识别表位的完整性。
