白色念珠菌烯醇化酶基因的克隆与核苷酸序列分析

Cloning and nucleotide sequence analysis of the Candida albicans enolase gene.

作者信息

Franklyn K M, Warmington J R

机构信息

School of Biomedical Sciences, Curtin University of Technology, Perth, Australia.

出版信息

FEMS Microbiol Lett. 1993 Jul 15;111(1):101-7. doi: 10.1111/j.1574-6968.1993.tb06368.x.

DOI:10.1111/j.1574-6968.1993.tb06368.x

PMID:8359671

Abstract

The complete nucleotide sequence of the coding region as well as the flanking non-coding region of Candida albicans enolase gene was determined. A continuous open reading frame of 1323 nucleotides with no introns was identified. The deduced amino acid sequence showed 87% similarity to the enolases from the yeast Saccharomyces cerevisiae. The two isoforms of enolase are encoded by two non-tandemly arrayed genes in S. cerevisiae. However, DNA hybridisation analysis indicates that in C. albicans enolase is encoded by a single gene. The position of the transcription start site, putative TATA box and polyadenylation signal of the C. albicans enolase gene have been identified. The location of these sequences are similar to those of the S. cerevisiae enolase genes.

摘要

测定了白色念珠菌烯醇化酶基因编码区以及侧翼非编码区的完整核苷酸序列。鉴定出一个1323个核苷酸的连续开放阅读框，无内含子。推导的氨基酸序列与酿酒酵母的烯醇化酶显示出87%的相似性。在酿酒酵母中，烯醇化酶的两种同工型由两个非串联排列的基因编码。然而，DNA杂交分析表明，在白色念珠菌中，烯醇化酶由单个基因编码。已确定白色念珠菌烯醇化酶基因的转录起始位点、假定的TATA框和聚腺苷酸化信号的位置。这些序列的位置与酿酒酵母烯醇化酶基因的位置相似。