Paris S, Monod M, Diaquin M, Lamy B, Arruda L K, Punt P J, Latgé J P
Unite de Mycologie, Institut Pasteur, Paris, France.
FEMS Microbiol Lett. 1993 Jul 15;111(1):31-6. doi: 10.1111/j.1574-6968.1993.tb06357.x.
The aspfI gene encoding a ribonucleotoxin, a putative virulence factor of Aspergillus fumigatus, was inactivated by gene disruption. Gene replacement through homologous recombination by the disrupted allele tagged by the hygromycin B resistance marker was performed by transformation of a pathogenic strain. One transformant with the disrupted aspfI gene failed to produce the ASPFI protein and was shown to be pathogenic for mice. We concluded that this ribotoxin is not a main factor in the colonization of the lung tissues by A. fumigatus.
编码核糖核酸毒素（烟曲霉一种假定的毒力因子）的aspfI基因通过基因破坏被灭活。通过用潮霉素B抗性标记物标记的破坏等位基因进行同源重组来替换基因，这是通过对致病菌株进行转化来完成的。一个aspfI基因被破坏的转化体无法产生ASPFI蛋白，并且对小鼠具有致病性。我们得出结论，这种核糖核酸毒素不是烟曲霉在肺组织中定殖的主要因素。
