Roy F, DeBlois C, Doillon C J
Laval University, Quebec, Canada.
J Biomed Mater Res. 1993 Mar;27(3):389-97. doi: 10.1002/jbm.820270312.
Repair of connective tissue involves interactions between growth factors (GFs) and extracellular matrix (ECM) molecules. On the other hand, biological biomaterials could be used to carry and deliver GFs to stimulate wound healing. In the present study, fibroblasts were cultivated in a serum-free culture medium onto collagen (type I), hyaluronic acid, chondroitin sulfate, fibronectin, or fibrin. FGF, EGF, or PDGF was incorporated within those substrates. Using immuno- and radiolabeling assays, the distribution of GFs within the ECM analogs was relatively uniform and GFs retained in substrates were dependent on the substrates. Fibroblast replication was determined by the incorporation of [3H]-thymidine and compared to control groups. On collagen or chondroitin sulfate, the incorporation of GFs did not significantly improve cell proliferation. On hyaluronic acid, the incorporation of FGF and PDGF enhanced cell replication at 48 h. The incorporation of PDGF in fibronectin enhanced cell replication. On fibrin, the incorporation of PDGF, EGF, and FGF significantly enhanced cell replication. However, cell replication on FGF-incorporated fibrin was higher by 48 h than that on fibrin in the presence of FGF-supplemented culture medium. Fibrin sustains the biological activity of GFs, FGF in particular, and can be a carrier for GF that stimulates cell replication.
结缔组织的修复涉及生长因子（GFs）与细胞外基质（ECM）分子之间的相互作用。另一方面，生物生物材料可用于携带和递送生长因子以刺激伤口愈合。在本研究中，将成纤维细胞在无血清培养基中培养在胶原蛋白（I型）、透明质酸、硫酸软骨素、纤连蛋白或纤维蛋白上。将FGF、EGF或PDGF掺入这些底物中。使用免疫和放射性标记测定法，生长因子在细胞外基质类似物中的分布相对均匀，并且保留在底物中的生长因子取决于底物。通过掺入[3H] - 胸腺嘧啶核苷来测定成纤维细胞的复制，并与对照组进行比较。在胶原蛋白或硫酸软骨素上，掺入生长因子并没有显著改善细胞增殖。在透明质酸上，掺入FGF和PDGF在48小时时增强了细胞复制。在纤连蛋白中掺入PDGF增强了细胞复制。在纤维蛋白上，掺入PDGF、EGF和FGF显著增强了细胞复制。然而，在掺入FGF的纤维蛋白上的细胞复制在48小时时比在补充有FGF的培养基存在下的纤维蛋白上的细胞复制更高。纤维蛋白维持生长因子的生物活性，特别是FGF，并且可以作为刺激细胞复制的生长因子的载体。
