Cartwright E J, Gordon M T, Freemont A J, Anderson D C, Sharpe P T
Department of Cell and Structural Biology, University of Manchester, England.
J Med Virol. 1993 Jun;40(2):133-41. doi: 10.1002/jmv.1890400210.
Using the technique of in situ hybridisation, we extended our initial studies of the occurrence of canine distemper and measles virus in Paget's disease. Bone samples from untreated patients and patients treated with 3-amino-hydroxypropylidene 1,1 bisphosphonate pamidronate (APD) were examined for canine distemper nucleocapsid (CDV-N), measles nucleocapsid (MV-N), respiratory syncytial virus fusion (RSV-F), and simian virus 5 haemoglutinin-neuraminidase (SV5-HN) sequences using 35S-labelled sense and antisense riboprobes. Only CDV-N mRNA was detected in osteoclasts, osteoblasts, and mononuclear cells. In untreated Paget's patients, 63.5% were found to have CDV sequences residing in bone cells, and 48.2% of those undergoing APD treatment were still found to have the viral sequences. RSV-F mRNA was only found in one patient. None of the other paramyxoviruses tested were detected. This study shows that CDV can infect a human host and may be an aetiological agent of Paget's disease of bone.
利用原位杂交技术，我们扩展了对佩吉特病中犬瘟热病毒和麻疹病毒存在情况的初步研究。使用35S标记的正义和反义核糖探针，对未经治疗的患者以及用3 - 氨基 - 羟丙基亚丙基 - 1,1 - 双膦酸帕米膦酸二钠（APD）治疗的患者的骨样本进行检测，以查找犬瘟热核衣壳（CDV - N）、麻疹核衣壳（MV - N）、呼吸道合胞病毒融合蛋白（RSV - F）和猿猴病毒5血凝素 - 神经氨酸酶（SV5 - HN）序列。仅在破骨细胞、成骨细胞和单核细胞中检测到CDV - N mRNA。在未经治疗的佩吉特病患者中，发现63.5%的患者骨细胞中存在CDV序列，在接受APD治疗的患者中，仍有48.2%被发现有病毒序列。仅在一名患者中发现了RSV - F mRNA。未检测到所测试的其他副粘病毒。这项研究表明，CDV可感染人类宿主，可能是骨佩吉特病的病因。
