Ralston S H, Digiovine F S, Gallacher S J, Boyle I T, Duff G W
Molecular Immunology Group, Northern General Hospital, Edinburgh, Scotland.
J Bone Miner Res. 1991 Nov;6(11):1243-8. doi: 10.1002/jbmr.5650061115.
It is widely believed that Paget's disease of bone is due to a "slow virus" infection of osteoclasts with one of the paramyxovirus group. Controversy continues to surround the identity of the virus involved, however, since at different times evidence has been presented implicating measles virus (MV), respiratory syncytial virus (RSV), and canine distemper virus (CDV) as putative infective agents. In this study we used the technique of reverse transcription and polymerase chain reaction (PCR) to screen for paramyxovirus sequences in ribonucleic acid (RNA) extracted from pagetic bone. We were able to detect viral amplification products of the appropriate size in RNA extracted from as few as 50 cells experimentally infected with a wide range of paramyxoviruses, including measles, canine distemper, parainfluenza 3, and respiratory syncytial virus, but we found no evidence of viral products in RNA extracts of affected bone from 10 consecutive patients with Paget's disease. This study fails to support the hypothesis that active infection with one of these or a related paramyxovirus is involved in the pathogenesis of Paget's disease.
人们普遍认为，骨佩吉特病是由于破骨细胞受到副粘病毒组中的一种“慢病毒”感染所致。然而，关于所涉及病毒的身份仍存在争议，因为在不同时期都有证据表明麻疹病毒（MV）、呼吸道合胞病毒（RSV）和犬瘟热病毒（CDV）可能是感染源。在本研究中，我们使用逆转录和聚合酶链反应（PCR）技术，从佩吉特病骨组织提取的核糖核酸（RNA）中筛选副粘病毒序列。我们能够在从实验性感染多种副粘病毒（包括麻疹、犬瘟热病毒、副流感3型和呼吸道合胞病毒）的少至50个细胞中提取的RNA中检测到合适大小的病毒扩增产物，但在连续10例佩吉特病患者的患骨RNA提取物中未发现病毒产物的证据。这项研究不支持这些或相关副粘病毒之一的活跃感染参与佩吉特病发病机制的假说。
