GABA- and glycine-like immunoreactivities in the cerebellum of the frog.

Immunocytochemistry was performed on consecutive semi-thin sections of frog cerebellum with antibodies raised against GABA and glycine. Somata of Purkinje cells and stellate cells were immunoreactive for GABA but not for glycine. In the granular layer some neurons were immunoreactive for GABA, fewer for glycine and even fewer for both GABA and glycine. Most of the GABA-positive neurons appear to be Golgi cells. Most of the glycine-positive neurons appear to be displaced cerebellar nucleus neurons. Compared with mammals true basket cells are missing, stellate cells are fewer in number and colocalization of GABA and glycine in Golgi neurons is encountered about ten times less often in the frog than in the rat.