Gray S T, Yun K, Motoori T, Kuys Y M
Department of Oral Medicine, University of Otago, Dunedin, New Zealand.
Am J Pathol. 1993 Sep;143(3):663-71.
Tumor invasion and metastasis are complex phenomena believed to be facilitated by the disruption of collagen and elastin fibers in the extracellular matrix. Interstitial collagenase gene expression was studied in colonic adenocarcinoma and adenoma using in situ hybridization. The data indicated that three cell types within the tumor stroma expressed collagenase transcripts; they were eosinophils, fibroblasts, and vascular endothelium. In all 12 adenocarcinomas, a high to moderate level of expression was seen in 1 to 5% of eosinophils and in occasional fibroblasts, whereas these cell types in non-neoplastic mucosa adjacent to tumor showed no detectable expression. Two adenocarcinomas showed expression in hyperplastic endothelium in vascularized granulation tissue. Two out of three adenomas showed expression in eosinophils and fibroblasts at a reduced level. Tissue inhibitor of metalloproteinase-1 gene expression was, however, negligible in all tissue examined. These results suggest that interstitial collagenase gene activation in the tumor stroma, especially eosinophils, may have an important role in tumor invasion and metastasis.
肿瘤侵袭和转移是复杂的现象，据信细胞外基质中胶原蛋白和弹性纤维的破坏会促进这一过程。使用原位杂交技术研究了结肠腺癌和腺瘤中组织间质胶原酶基因的表达。数据表明，肿瘤基质中的三种细胞类型表达胶原酶转录本，它们是嗜酸性粒细胞、成纤维细胞和血管内皮细胞。在所有12例腺癌中，1%至5%的嗜酸性粒细胞和偶尔的成纤维细胞呈现高至中等水平的表达，而肿瘤旁非肿瘤性黏膜中的这些细胞类型未检测到表达。两例腺癌在血管化肉芽组织的增生内皮细胞中出现表达。三例腺瘤中有两例在嗜酸性粒细胞和成纤维细胞中呈现较低水平的表达。然而，在所有检测的组织中，金属蛋白酶组织抑制剂-1基因的表达可忽略不计。这些结果表明，肿瘤基质尤其是嗜酸性粒细胞中的组织间质胶原酶基因激活可能在肿瘤侵袭和转移中起重要作用。
