Okada T, Morikawa Y
Department of Veterinary Anatomy, College of Agriculture, University of Osaka Prefecture, Japan.
Anat Rec. 1993 Jul;236(3):563-7. doi: 10.1002/ar.1092360316.
Development of the glomerulus in fetal kidney was studied morphometrically following ligation of both ureters of pregnant rats. The ligation was performed on days 17, 19, and 21 of gestation, and autopsy was done 24 hr after each operation. The percentage volumes of the five glomerular components (epithelial cells, capillary, mesangium, glomerular basement membrane, and Bowman's space) were determined by point counting, and the surface area of glomerular basement membrane per unit volume of glomerulus was calculated by intercept counting. On fetal days 18, 20, and 22, percentage volume of Bowman's space in the fetuses from the ligated mothers was significantly smaller than that in the fetuses from sham-ligated mothers. On fetal days 20 and 22, surface area of glomerular basement membrane and glomerular capillary length per unit volume of glomerulus and percentage of glomerular capillary volume in the fetuses from the ligated mothers were significantly larger than those in the fetuses from sham-ligated mothers. These results suggest that maternal bilateral ureteral ligation induces an increase in filtration area of glomerulus in fetal kidney when the fetal kidney is functional.
在妊娠大鼠双侧输尿管结扎后，对胎儿肾脏中肾小球的发育进行了形态计量学研究。在妊娠第17、19和21天进行结扎，并在每次手术后24小时进行尸检。通过点计数确定五种肾小球成分（上皮细胞、毛细血管、系膜、肾小球基底膜和鲍曼间隙）的体积百分比，并通过截距计数计算每单位肾小球体积的肾小球基底膜表面积。在胎儿第18、20和22天，结扎母亲的胎儿中鲍曼间隙的体积百分比显著小于假结扎母亲的胎儿。在胎儿第20和22天，结扎母亲的胎儿中每单位肾小球体积的肾小球基底膜表面积、肾小球毛细血管长度以及肾小球毛细血管体积百分比均显著大于假结扎母亲的胎儿。这些结果表明，当胎儿肾脏具有功能时，母体双侧输尿管结扎会导致胎儿肾脏中肾小球滤过面积增加。
