Okada T, Morikawa Y, Kiso Y, Sasaki F
Department of Veterinary Anatomy, College of Agriculture, Osaka Prefecture University, Japan.
Anat Rec. 1997 Oct;249(2):181-6. doi: 10.1002/(SICI)1097-0185(199710)249:2<181::AID-AR4>3.0.CO;2-0.
The present study was designed to clarify development of filtration property of fetal renal glomerulus when maternal kidney is dysfunctional.
Maternal bilateral ureteral ligation was performed on days 17, 19, and 21 of pregnancy. One day after each operation, cationized ferritin (CF), native ferritin (NF), and horseradish peroxidase (HRP) were injected, respectively, in the fetuses. Distribution of the tracers in the fetal kidneys was investigated electron microscopically.
On fetal day 20, clustered CF particles were present in the laminae rarae interna and externa of the glomerular basement membrane of the fetuses from ureter-ligated mothers, while the clusters were arrayed in three to four layers in that of the fetuses from sham-ligated ones. On fetal day 22, a relatively large amount of CF particles was present in the lamina rara externa in the fetuses from ureter-ligated mothers when compared to that in the age-matched control fetuses. On fetal days 20 and 22, the number of NF particles was decreased, and shortening of the time for filtration of HRP through the glomerular basement membrane was observed in the fetuses from the ligated mothers.
These results suggest that dysfunction of maternal kidneys causes accelerated formation of fetal glomerular basement membrane and stimulates glomerular function in filtration in fetal rat kidney.
本研究旨在阐明母体肾脏功能不全时胎儿肾小球滤过特性的发育情况。
在妊娠第17、19和21天对母体进行双侧输尿管结扎。每次手术后一天，分别向胎儿注射阳离子铁蛋白（CF）、天然铁蛋白（NF）和辣根过氧化物酶（HRP）。通过电子显微镜研究示踪剂在胎儿肾脏中的分布。
在胎儿第20天时，输尿管结扎母体的胎儿肾小球基底膜内、外疏松层出现簇状CF颗粒，而假结扎母体的胎儿肾小球基底膜内CF颗粒呈三到四层排列。在胎儿第22天时，与年龄匹配的对照胎儿相比，输尿管结扎母体的胎儿肾小球基底膜外疏松层存在相对大量的CF颗粒。在胎儿第20天和22天时，结扎母体的胎儿NF颗粒数量减少，且观察到HRP通过肾小球基底膜的滤过时间缩短。
这些结果表明，母体肾脏功能不全会导致胎儿肾小球基底膜形成加速，并刺激胎儿大鼠肾脏的肾小球滤过功能。
