Adachi S, Kawamura K, Takemoto K
Department of Public Health, Saitama Medical School, Japan.
Cancer Res. 1993 Sep 15;53(18):4153-5.
Male Sprague-Dawley rats were exposed to conditioned emotional stimuli in a communication box, which is much more psychologically conditioned stress than the commonly used restraint and water immersion, to investigate the induction of oxidative DNA damage by psychological stress. Significantly higher levels of 8-hydroxy-2'-deoxyguanosine in rat liver nuclear DNA than in the controls [1.46 +/- 0.19 (SD) 8-hydroxy-2'-deoxyguanosine/10(5) deoxyguanosine] were detected immediately after the second (1.90 +/- 0.27, P < 0.01), third (3.10 +/- 0.94, P < 0.01), and fourth exposure (2.95 +/- 1.17, P < 0.01) to conditioned emotional stimuli. This is the first evidence that oxidative damage to nuclear DNA is induced by psychological stress.
将雄性斯普拉格-道利大鼠置于一个交流箱中，使其暴露于条件性情绪刺激下，相较于常用的束缚和水浸法，这种刺激在心理上造成的应激要大得多，以此来研究心理应激对氧化性DNA损伤的诱导作用。在第二次（1.90±0.27，P<0.01）、第三次（3.10±0.94，P<0.01）和第四次暴露于条件性情绪刺激后，立即检测到大鼠肝细胞核DNA中8-羟基-2'-脱氧鸟苷的水平显著高于对照组[1.46±0.19（标准差）8-羟基-2'-脱氧鸟苷/10⁵脱氧鸟苷]。这是心理应激可诱导细胞核DNA发生氧化性损伤的首个证据。
