Gałazyn-Sidorczuk Małgorzata, Brzóska Małgorzata M, Jurczuk Maria, Moniuszko-Jakoniuk Janina
Department of Toxicology, Medical University of Białystok, Białystok, Poland.
Chem Biol Interact. 2009 Jun 15;180(1):31-8. doi: 10.1016/j.cbi.2009.01.014. Epub 2009 Feb 10.
The study was aimed to estimate whether rat's exposure to cadmium (Cd; 50mg/l in drinking water for 12 weeks) and/or ethanol (EtOH; 5g/kg b.wt./24h p.o. for 12 weeks), noted by us to induce oxidative stress and stimulate lipid peroxidation, can cause oxidative damage to proteins and DNA, and whether and to what extent the effects of co-exposure differ from those observed under the treatment with each substance alone. Protein carbonyl groups (PC) and protein thiol groups (PSH) in the serum, liver and kidney, as markers of oxidative protein damage, and 8-hydroxy-2'-deoxyguanosine (8-OHdG) in the serum, as a marker of DNA oxidation, were determined. The exposure to Cd or/and EtOH led to oxidative protein damage (increased PC and decreased PSH concentrations in the serum and/or liver), and to DNA oxidation (increased 8-OHdG concentration in the serum). The effects were more advanced at the co-exposure than at the treatment with each substance alone. The more serious damage to proteins and DNA at the co-exposure to Cd and EtOH seems to be the effect of independent action of both xenobiotics. The results of the present paper together with our recent findings in the same rats seem to indicate that at co-exposure to Cd and EtOH proteins and DNA may be more vulnerable to oxidation than lipids. The paper is the first report suggesting that excessive EtOH consumption during exposure to Cd may increase the risk of health damage via enhancing protein and DNA oxidation.
本研究旨在评估大鼠暴露于镉（Cd；饮用水中50mg/l，持续12周）和/或乙醇（EtOH；5g/kg体重/24小时，经口给药，持续12周）——我们注意到这两种物质会诱导氧化应激并刺激脂质过氧化——是否会对蛋白质和DNA造成氧化损伤，以及联合暴露的影响与单独使用每种物质时观察到的影响是否不同以及在何种程度上不同。测定了血清、肝脏和肾脏中的蛋白质羰基（PC）和蛋白质巯基（PSH），作为氧化蛋白质损伤的标志物，以及血清中的8-羟基-2'-脱氧鸟苷（8-OHdG），作为DNA氧化的标志物。暴露于Cd或/和EtOH会导致氧化蛋白质损伤（血清和/或肝脏中PC增加，PSH浓度降低）以及DNA氧化（血清中8-OHdG浓度增加）。联合暴露时的影响比单独使用每种物质时更严重。同时暴露于Cd和EtOH时对蛋白质和DNA造成的更严重损伤似乎是两种异源生物独立作用的结果。本文的结果以及我们最近在同一只大鼠身上的发现似乎表明，同时暴露于Cd和EtOH时，蛋白质和DNA可能比脂质更容易受到氧化损伤。本文是首份表明在接触Cd期间过量饮用EtOH可能通过增强蛋白质和DNA氧化而增加健康损害风险的报告。
