Bounous G, Baruchel S, Falutz J, Gold P
Department of Surgery, Montreal General Hospital, Quebec.
Clin Invest Med. 1993 Jun;16(3):204-9.
On the basis of numerous animal experiments, a pilot study was undertaken to evaluate the effect of undenatured, biologically active, dietary whey protein in 3 HIV-seropositive individuals over a period of 3 months. Whey protein concentrate was prepared so that the most thermosensitive proteins, such as serum albumin which contains 6 glutamylcysteine groups, would be in undenatured form. Whey protein powder dissolved in a drink of the patient's choice was drunk cold in quantities that were increased progressively from 8.4 to 39.2 g per day. Patients took whey proteins without adverse side effects. In the 3 patients whose body weight had been stable in the preceding 2 months, weight gain increased progressively between 2 and 7 kg, with 2 of the patients reaching ideal body weight. Serum proteins, including albumin, remained unchanged and within normal range, indicating that protein replenishment per se was not likely the cause of increased body weight. The glutathione content of the blood mononuclear cells was, as expected, below normal values in all patients at the beginning of the study. Over the 3-month period, glutathione levels increased in all 3 cases. In conclusion, these preliminary data indicate that, in patients who maintain an adequate total caloric intake, the addition of "bioactive" whey protein concentrate as a significant portion of total protein intake increases body weight and shows elevation of glutathione (GSH) content of mononuclear cells toward normal levels. This pilot study will serve as a basis for a much larger clinical trial.
基于大量动物实验，开展了一项初步研究，以评估未变性的、具有生物活性的膳食乳清蛋白对3名HIV血清反应阳性个体在3个月期间的影响。制备了浓缩乳清蛋白，以便使最热敏的蛋白质，如含有6个谷氨酰半胱氨酸基团的血清白蛋白，处于未变性状态。将乳清蛋白粉溶解在患者选择的饮品中，冷饮，每天摄入量从8.4克逐渐增加到39.2克。患者服用乳清蛋白未出现不良副作用。在之前两个月体重一直稳定的3名患者中，体重增加在2至7千克之间逐渐增加，其中2名患者达到理想体重。包括白蛋白在内的血清蛋白保持不变且在正常范围内，这表明蛋白质补充本身不太可能是体重增加的原因。在研究开始时，所有患者血液单核细胞中的谷胱甘肽含量均低于正常值。在3个月期间，所有3例患者的谷胱甘肽水平均有所升高。总之，这些初步数据表明，在保持足够总热量摄入的患者中，添加“生物活性”浓缩乳清蛋白作为总蛋白摄入量的重要组成部分会增加体重，并使单核细胞的谷胱甘肽（GSH）含量升高至正常水平。这项初步研究将作为一项更大规模临床试验的基础。
