Kruse A, Neustock P, Reuter M, Kirchner H
Institute of Immunology and Transfusion Medicine, University of Lübeck Medical School, Germany.
J Interferon Res. 1993 Jun;13(3):221-5. doi: 10.1089/jir.1993.13.221.
We investigated T-cell surface antigen expression and the production of interferon-gamma (IFN-gamma) and interleukin-2 (IL-2) by human cord and adult blood cells. Cord blood lymphocytes stimulated with Staphylococcus enterotoxin B (SEB) released only minimal titers of IFN-gamma and IL-2 in comparison with adult blood lymphocytes. Systematic analyses of several cell-surface molecules on untreated cord blood and adult blood T cells demonstrated a significant reduction of CD8+ subset numbers in cord blood. Furthermore, cord blood lymphocytes did not express the memory T-cell marker CD45RO. No differences were found in gamma/delta T-cell receptor (TCR), CD5, and CD25 antigen expression between untreated cord blood and adult blood. However, cord blood mononuclear cells showed a decreased CD25 antigen expression in comparison to those of adults following activation with SEB. These observations suggest that the decrease in the proportion of some T-cell subsets and the absence of CD45RO memory T cells may be responsible for the diminished lymphokine production of cord blood lymphocytes.
我们研究了人脐带血和成人血细胞的T细胞表面抗原表达以及干扰素-γ（IFN-γ）和白细胞介素-2（IL-2）的产生。与成人血淋巴细胞相比，用金黄色葡萄球菌肠毒素B（SEB）刺激的脐带血淋巴细胞仅释放极低滴度的IFN-γ和IL-2。对未经处理的脐带血和成人血T细胞上几种细胞表面分子的系统分析表明，脐带血中CD8 +亚群数量显著减少。此外，脐带血淋巴细胞不表达记忆T细胞标志物CD45RO。未经处理的脐带血和成人血之间在γ/δ T细胞受体（TCR）、CD5和CD25抗原表达上未发现差异。然而，与用SEB激活后的成人相比，脐带血单个核细胞显示出CD25抗原表达降低。这些观察结果表明，某些T细胞亚群比例的降低和CD45RO记忆T细胞的缺失可能是脐带血淋巴细胞淋巴因子产生减少的原因。
