Lambert S, Bennett V
Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710.
J Neurosci. 1993 Sep;13(9):3725-35. doi: 10.1523/JNEUROSCI.13-09-03725.1993.
Isoforms of ankyrin (ankyrinR) are expressed in both the erythrocyte and the brain. Four cDNAs representing regulatory domains of ankyrinR expressed in the rat spleen and brain were cloned and sequenced. These different cDNAs were found to result from tissue-specific alternative mRNA processing of the ankyrinR regulatory domain. One of the isolated cDNAs was used to develop an antibody to brain isoforms of ankyrinR, and this antibody was used to study the localization of ankyrinR in the rat brain. The protein was found to be widely expressed in neurons of the metencephalon but limited to a discrete subset of neurons in the rat forebrain. In the thalamus and areas of the basal ganglia, these neurons were grouped in defined nuclei, whereas in the cortex, hippocampus, and caudate putamen they appeared as isolated cells distributed randomly throughout these structures. A similar study using an antibody raised against erythrocyte spectrin (beta R) showed a comparable localization to that of ankyrinR. Both proteins were expressed late in the developing rat brain, as part of the maturation stage of neural development. These data suggest a specific role for these erythrocyte structural proteins in the postmitotic development of a subset of neurons in the rat brain.
锚蛋白异构体（ankyrinR）在红细胞和大脑中均有表达。克隆并测序了代表大鼠脾脏和大脑中表达的锚蛋白R调节域的四个cDNA。发现这些不同的cDNA是由锚蛋白R调节域的组织特异性可变mRNA加工产生的。其中一个分离出的cDNA被用于制备针对锚蛋白R脑异构体的抗体，该抗体用于研究锚蛋白R在大鼠脑中的定位。发现该蛋白在后脑神经元中广泛表达，但仅限于大鼠前脑的离散神经元亚群。在丘脑和基底神经节区域，这些神经元聚集在特定的核中，而在皮质、海马体和尾状壳核中，它们表现为随机分布在这些结构中的孤立细胞。一项使用针对红细胞血影蛋白（βR）产生的抗体进行的类似研究显示，其定位与锚蛋白R相似。这两种蛋白在大鼠脑发育后期表达，是神经发育成熟阶段的一部分。这些数据表明这些红细胞结构蛋白在大鼠脑一部分神经元的有丝分裂后发育中具有特定作用。
