重组步移：通过同源重组从酵母人工染色体混合文库中进行克隆的遗传筛选。

Miller A M, Savinelli E A, Couture S M, Hannigan G M, Han Z, Selden R F, Treco D A

Transkaryotic Therapies, Inc., Cambridge, MA 02139.

Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8118-22. doi: 10.1073/pnas.90.17.8118.

Recombination walking is based on the genetic selection of specific human clones from a yeast artificial chromosome (YAC) library by homologous recombination. The desired clone is selected from a pooled (unordered) YAC library, eliminating labor-intensive steps typically used in organizing and maintaining ordered YAC libraries. Recombination walking represents an efficient approach to library screening and is well suited for chromosome-walking approaches to the isolation of genes associated with common diseases.

重组步移是基于通过同源重组从酵母人工染色体（YAC）文库中对特定人类克隆进行遗传筛选。所需克隆是从混合（无序）YAC文库中选出的，省去了通常用于构建和维护有序YAC文库的劳动密集型步骤。重组步移是一种高效的文库筛选方法，非常适合用于通过染色体步移法分离与常见疾病相关的基因。