在半限定培养基中培养布氏锥虫、马媾疫锥虫、伊氏锥虫、罗德西亚锥虫和冈比亚锥虫的动物感染性形态。
Cultivation in a semi-defined medium of animal infective forms of Trypanosoma brucei, T. equiperdum, T. evansi, T. rhodesiense and T. gambiense.
作者信息
Baltz T, Baltz D, Giroud C, Crockett J
出版信息
EMBO J. 1985 May;4(5):1273-7. doi: 10.1002/j.1460-2075.1985.tb03772.x.
Abstract
A semi-defined medium for the cultivation of bloodstream forms of the African trypanosome brucei subgroup was developed. Out of 14 different strains tested, 10 could be cultured including Trypanosoma brucei, T. equiperdum, T. evansi, T. rhodesiense and T. gambiense. The presence of a reducing agent (2-mercaptoethanol or thioglycerol) was found to be essential for growth. The standard medium consisted of Hepes buffered minimum essential medium with Earle's salts supplemented with 0.2 mM 2-mercaptoethanol, 2 mM pyruvate and 10% inactivated serum either from rabbit (T. brucei, T. equiperdum, T. evansi and T. rhodesiense) or human (T. gambiense). Although a general medium could be defined for the long-term maintenance of trypanosome cultures, the initiation to culture nevertheless required particular conditions for the different strains. The cultured trypanosomes had all the characteristics of the in vivo bloodstream forms including: morphology, infectivity, antigenic variation and glucose metabolism.
摘要
开发了一种用于培养非洲布氏锥虫亚组血流形式的半限定培养基。在测试的14种不同菌株中，10种能够培养，包括布氏锥虫、马媾疫锥虫、伊氏锥虫、罗德西亚锥虫和冈比亚锥虫。发现还原剂（2-巯基乙醇或硫代甘油）的存在对生长至关重要。标准培养基由含有Earle盐的Hepes缓冲最低限度基本培养基组成，补充有0.2 mM 2-巯基乙醇、2 mM丙酮酸盐和10%来自兔（布氏锥虫、马媾疫锥虫、伊氏锥虫和罗德西亚锥虫）或人（冈比亚锥虫）的灭活血清。尽管可以定义一种通用培养基用于锥虫培养物的长期维持，但不同菌株的培养起始仍需要特定条件。培养的锥虫具有体内血流形式的所有特征，包括：形态、感染性、抗原变异和葡萄糖代谢。
相似文献
1
Cultivation in a semi-defined medium of animal infective forms of Trypanosoma brucei, T. equiperdum, T. evansi, T. rhodesiense and T. gambiense.在半限定培养基中培养布氏锥虫、马媾疫锥虫、伊氏锥虫、罗德西亚锥虫和冈比亚锥虫的动物感染性形态。
EMBO J. 1985 May;4(5):1273-7. doi: 10.1002/j.1460-2075.1985.tb03772.x.
2
In vitro cultivation of bloodstream forms of Trypanosoma brucei, T. rhodesiense, and T. gambiense.布氏锥虫、罗德西亚锥虫和冈比亚锥虫血液形态的体外培养。
J Protozool. 1981 Nov;28(4):470-9. doi: 10.1111/j.1550-7408.1981.tb05322.x.
3
Evaluation of the in vitro transformation technique to distinguish Trypanosoma evansi from cyclically transmitted Trypanozoon stocks.评估体外转化技术以区分伊氏锥虫与循环传播的锥虫种群。
Trop Med Parasitol. 1993 Jun;44(2):108-10.
4
Trypanosoma brucei gambiense: HMI-9 medium containing methylcellulose and human serum supports the continuous axenic in vitro propagation of the bloodstream form.布氏冈比亚锥虫：含有甲基纤维素和人血清的 HMI-9 培养基支持血液形式的连续体外无细胞培养。
Exp Parasitol. 2011 Jul;128(3):285-90. doi: 10.1016/j.exppara.2011.02.018. Epub 2011 Feb 24.
5
Long-term culture and cloning system for Trypanosoma brucei gambiense bloodstream forms in semi-defined medium in vitro.
Parasitol Res. 1989;76(2):93-7. doi: 10.1007/BF00930828.
6
Cultivation of the life cycle stages of Trypanosoma brucei sspp.布氏锥虫亚种生命周期各阶段的培养
Acta Trop. 1988 Mar;45(1):33-43.
7
Growth and antigenic variation of Trypanosoma brucei, T. rhodesiense and T. gambiense in subcutaneous millipore chambers.布氏锥虫、罗德西亚锥虫和冈比亚锥虫在皮下微孔小室中的生长及抗原变异
Trans R Soc Trop Med Hyg. 1985;79(1):24-8. doi: 10.1016/0035-9203(85)90224-x.
8
Cultural and physiological observations on Trypanosoma rhodesiense and Trypanosoma gambiense. 1949.关于罗德西亚锥虫和冈比亚锥虫的文化与生理学观察。1949年。
J Parasitol. 2001 Aug;87(4):714-7. doi: 10.1645/0022-3395(2001)087[0714:CAPOOT]2.0.CO;2.
9
Differentiation of Trypanosoma brucei, T. rhodesiense, and T. gambiense by the indirect fluorescent antibody test.通过间接荧光抗体试验鉴别布氏锥虫、罗德西亚锥虫和冈比亚锥虫。
Bull World Health Organ. 1973;48(4):401-7.
10
An effective axenic culture system for Trypanosoma brucei rhodesiense blood stream forms in vitro.一种用于布氏罗得西亚锥虫血流型体外培养的有效无菌培养系统。
Southeast Asian J Trop Med Public Health. 1990 Dec;21(4):564-7.
引用本文的文献
1
Antiproliferative and Trypanocidal Activity of Ivermectin Bioconjugates.伊维菌素生物共轭物的抗增殖和杀锥虫活性
ACS Omega. 2025 Jun 18;10(25):27380-27392. doi: 10.1021/acsomega.5c02998. eCollection 2025 Jul 1.
2
Spiroplasma endosymbiont reduction of host lipid synthesis and Stomoxyn-like peptide contribute to trypanosome resistance in the tsetse fly Glossina fuscipes.共生螺旋体对宿主脂质合成的减少以及类Stomoxyn肽有助于采采蝇舌蝇对锥虫产生抗性。
PLoS Pathog. 2025 Jan 31;21(1):e1012692. doi: 10.1371/journal.ppat.1012692. eCollection 2025 Jan.
3
Increased Trypanocidal Activity of the Salinomycin Derivative Ironomycin Is Due to ROS Production and Iron Uptake Impairment.盐霉素衍生物铁霉素杀锥虫活性增强是由于活性氧生成和铁摄取受损。
Molecules. 2024 Nov 27;29(23):5597. doi: 10.3390/molecules29235597.
4
endosymbiont reduction of host lipid synthesis and Stomoxyn-like peptide contribute to trypanosome resistance in the tsetse fly .内共生体对宿主脂质合成的减少以及类Stomoxyn肽有助于采采蝇对锥虫的抗性。
bioRxiv. 2024 Oct 24:2024.10.24.620045. doi: 10.1101/2024.10.24.620045.
5
Venturicidin A affects the mitochondrial membrane potential and induces kDNA loss in .文替西定 A 影响线粒体膜电位并诱导. 的 kDNA 丢失。
Antimicrob Agents Chemother. 2024 Jul 9;68(7):e0167123. doi: 10.1128/aac.01671-23. Epub 2024 Jun 13.
6
A Sample-Centric and Knowledge-Driven Computational Framework for Natural Products Drug Discovery.一种以样本为中心且知识驱动的天然产物药物发现计算框架。
ACS Cent Sci. 2024 Feb 20;10(3):494-510. doi: 10.1021/acscentsci.3c00800. eCollection 2024 Mar 27.
7
Design, Synthesis, and Antiprotozoal Evaluation of New Promising 2,9-[(substituted-aminomethyl)]-4,7-phenyl-1,10-phenanthroline Derivatives, a Potential Alternative Scaffold to Drug Efflux.新型有前景的2,9-[(取代氨基甲基)]-4,7-苯基-1,10-菲咯啉衍生物的设计、合成及抗寄生虫评估，一种药物外排的潜在替代支架
Pathogens. 2022 Nov 13;11(11):1339. doi: 10.3390/pathogens11111339.
8
Efficient Oxidative Dearomatisations of Substituted Phenols Using Hypervalent Iodine (III) Reagents and Antiprotozoal Evaluation of the Resulting Cyclohexadienones against and Strain NF54.使用高价碘（III）试剂高效氧化取代苯酚，并对所得环己二烯酮进行抗原生动物评估，以测试其对 和 株 NF54 的抑制活性。
Molecules. 2022 Oct 4;27(19):6559. doi: 10.3390/molecules27196559.
9
Improving Aqueous Solubility and In Vitro Pharmacokinetic Properties of the 3-Nitroimidazo[1,2-]pyridine Antileishmanial Pharmacophore.改善3-硝基咪唑并[1,2 -]吡啶抗利什曼原虫药效基团的水溶性和体外药代动力学性质。
Pharmaceuticals (Basel). 2022 Aug 13;15(8):998. doi: 10.3390/ph15080998.
10
Discovery of Ircinianin Lactones B and C-Two New Cyclic Sesterterpenes from the Marine Sponge .发现 Ircinianin 内酯 B 和 C-两种新型海洋海绵中环二萜
Mar Drugs. 2022 Aug 19;20(8):532. doi: 10.3390/md20080532.
本文引用的文献
1
Absence of kinetoplast DNA in a late antigenic variant of Trypanosoma equiperdum.
Mol Biochem Parasitol. 1980 Apr;1(2):97-105. doi: 10.1016/0166-6851(80)90004-3.
2
In vitro cultivation of bloodstream forms of Trypanosoma brucei, T. rhodesiense, and T. gambiense.布氏锥虫、罗德西亚锥虫和冈比亚锥虫血液形态的体外培养。
J Protozool. 1981 Nov;28(4):470-9. doi: 10.1111/j.1550-7408.1981.tb05322.x.
3
Inhibition of glutathione synthesis as a chemotherapeutic strategy for trypanosomiasis.抑制谷胱甘肽合成作为治疗锥虫病的化疗策略。
J Exp Med. 1981 Mar 1;153(3):720-5. doi: 10.1084/jem.153.3.720.
4
Primary murine bone marrow cultures support continuous growth of infectious human trypanosomes.
Science. 1983 Apr 22;220(4595):421-3. doi: 10.1126/science.6836284.
5
Purine metabolism in the bloodstream forms of Trypanosoma gambiense and Trypanosoma rhodesiense.布氏锥虫和罗德西亚锥虫血液形式中的嘌呤代谢。
Biochim Biophys Acta. 1982 Nov 24;719(2):223-31. doi: 10.1016/0304-4165(82)90092-7.
6
Serological cross-reactions among trypanosome variable antigen isotypes of the subgenus Trypanozoon.锥虫亚属锥虫可变抗原同型之间的血清学交叉反应。
Ann Soc Belg Med Trop. 1982 Mar;62(1):25-39.
7
[Comparative immunoelectrophoretic analysis of culture and bloodstream forms of "trypanosoma" (Trypanozoon) brucei gambiense". Demonstration of stage and antigenic type specific antigens (author's transl)].布氏冈比亚锥虫（Trypanozoon）“锥虫”培养型和血流型的比较免疫电泳分析。阶段和抗原型特异性抗原的证明（作者译）
Ann Immunol (Paris). 1975 Jan;126(1):45-50.
8
Pedigrees of the Edinburgh Trypanosoma (Trypanozoon) antigenic types (ETat).爱丁堡锥虫（布氏锥虫）抗原型（ETat）的系谱
Trans R Soc Trop Med Hyg. 1975;69(2):205-8. doi: 10.1016/0035-9203(75)90156-x.
9
An approach to the development of new drugs for African trypanosomiasis.一种开发治疗非洲锥虫病新药的方法。
J Exp Med. 1978 Aug 1;148(2):569-79. doi: 10.1084/jem.148.2.569.
10
Antigenic variation in trypanosomes.锥虫的抗原变异
Nature. 1978 Jun 22;273(5664):613-7. doi: 10.1038/273613a0.
Zotero 插件