Baltz T, Baltz D, Giroud C, Crockett J
EMBO J. 1985 May;4(5):1273-7. doi: 10.1002/j.1460-2075.1985.tb03772.x.
A semi-defined medium for the cultivation of bloodstream forms of the African trypanosome brucei subgroup was developed. Out of 14 different strains tested, 10 could be cultured including Trypanosoma brucei, T. equiperdum, T. evansi, T. rhodesiense and T. gambiense. The presence of a reducing agent (2-mercaptoethanol or thioglycerol) was found to be essential for growth. The standard medium consisted of Hepes buffered minimum essential medium with Earle's salts supplemented with 0.2 mM 2-mercaptoethanol, 2 mM pyruvate and 10% inactivated serum either from rabbit (T. brucei, T. equiperdum, T. evansi and T. rhodesiense) or human (T. gambiense). Although a general medium could be defined for the long-term maintenance of trypanosome cultures, the initiation to culture nevertheless required particular conditions for the different strains. The cultured trypanosomes had all the characteristics of the in vivo bloodstream forms including: morphology, infectivity, antigenic variation and glucose metabolism.
开发了一种用于培养非洲布氏锥虫亚组血流形式的半限定培养基。在测试的14种不同菌株中，10种能够培养，包括布氏锥虫、马媾疫锥虫、伊氏锥虫、罗德西亚锥虫和冈比亚锥虫。发现还原剂（2-巯基乙醇或硫代甘油）的存在对生长至关重要。标准培养基由含有Earle盐的Hepes缓冲最低限度基本培养基组成，补充有0.2 mM 2-巯基乙醇、2 mM丙酮酸盐和10%来自兔（布氏锥虫、马媾疫锥虫、伊氏锥虫和罗德西亚锥虫）或人（冈比亚锥虫）的灭活血清。尽管可以定义一种通用培养基用于锥虫培养物的长期维持，但不同菌株的培养起始仍需要特定条件。培养的锥虫具有体内血流形式的所有特征，包括：形态、感染性、抗原变异和葡萄糖代谢。
