Moser M, Crameri R, Menz G, Suter M
Swiss Institute of Allergy and Asthma Research (SIAF), Davos-Platz.
Agents Actions Suppl. 1993;43:131-7. doi: 10.1007/978-3-0348-7324-6_11.
A. fumigatus allergen I/a (rAsp f I/a) was produced in Escherichia coli, affinity purified by Ni(2+)-chelate chromatography and refolded. rAsp f I/a binds IgE of A. fumigatus-allergic individuals as shown by ELISA, dot blots and Western blots. In addition, rAsp I/a elicited positive immediate type I skin reactions in individuals allergic to A. fumigatus but not in control individuals. Hence, rAsp f I/a can be used as a standardized antigen/allergen for an improved serological and clinical diagnosis of A. fumigatus-associated diseases.
