与脱辅基肌红蛋白结合的荧光探针的纳秒级衰变研究。
Nanosecond decay studies of a fluorescence probe bound to apomyoglobin.
作者信息
Gafni A, DeToma R P, Manrow R E, Brand L
出版信息
Biophys J. 1977 Feb;17(2):155-68. doi: 10.1016/S0006-3495(77)85633-6.
Abstract
Excited state interactions of N-(p-tolyl)-2-aminonaphthalene-6-sulfonate (2, 6 p-TNS) bound to apomyoglobin were studied by nanosecond time-resolved emission spectroscopy. A dynamic interaction of the excited dye molecule with its binding site, associated with a significant change in the emission energy with time, was observed. The decay kinetics were found to be complex and consistent with the kinetic model for solvent relaxation as proposed by Bakhshiev et al. (Opt. Spectrosc. 21:307. 1966). The behavior of 2, 6 p-TNS bound to apomyoglobin was found to be qualitatively similar to that of the dye dissolved in a viscous solvent such as glycerol or adsorbed to egg lecithin vesicles. The detailed information obtained by following the changes in emission spectra of fluorescent probes on the nanosecond time scale leads to a better understanding of their interactions with biological systems.
摘要
通过纳秒时间分辨发射光谱研究了与脱辅基肌红蛋白结合的N-（对甲苯基）-2-氨基萘-6-磺酸盐（2,6 p-TNS）的激发态相互作用。观察到激发态染料分子与其结合位点的动态相互作用，这与发射能量随时间的显著变化有关。发现衰减动力学很复杂，并且与Bakhshiev等人（《光学光谱学》21:307. 1966）提出的溶剂弛豫动力学模型一致。发现与脱辅基肌红蛋白结合的2,6 p-TNS的行为在定性上类似于溶解在甘油等粘性溶剂中或吸附在卵磷脂囊泡上的染料的行为。通过在纳秒时间尺度上跟踪荧光探针发射光谱的变化获得的详细信息，有助于更好地理解它们与生物系统的相互作用。
相似文献
1
Nanosecond decay studies of a fluorescence probe bound to apomyoglobin.与脱辅基肌红蛋白结合的荧光探针的纳秒级衰变研究。
Biophys J. 1977 Feb;17(2):155-68. doi: 10.1016/S0006-3495(77)85633-6.
2
Analysis of heterogeneous fluorescence decays in proteins. Using fluorescence lifetime of 8-anilino-1-naphthalenesulfonate to probe apomyoglobin unfolding at equilibrium.蛋白质中异质荧光衰减的分析。利用8-苯胺基-1-萘磺酸盐的荧光寿命探测脱辅基肌红蛋白在平衡状态下的去折叠过程。
Biochim Biophys Acta. 2006 Jul;1760(7):1125-37. doi: 10.1016/j.bbagen.2006.02.019. Epub 2006 Mar 31.
3
Nanosecond time-resolved emission spectroscopy of a fluorescence probe adsorbed to L-alpha-egg lecithin vesicles.吸附于L-α-卵磷脂囊泡的荧光探针的纳秒时间分辨发射光谱。
Biophys J. 1976 Jun;16(6):571-83. doi: 10.1016/S0006-3495(76)85712-8.
4
5
Red-edge excitation of fluorescence and dynamic properties of proteins and membranes.蛋白质和膜的荧光红边激发及动态特性
Biochemistry. 1984 Jun 19;23(13):3013-21. doi: 10.1021/bi00308a026.
6
Degree of dissociation of apohemoglobin studied by nano-second fluorescence-polarization technique.通过纳秒荧光偏振技术研究脱辅基血红蛋白的解离度。
Biochim Biophys Acta. 1975 May 30;393(1):10-4. doi: 10.1016/0005-2795(75)90210-x.
7
Characterization of the interactions of fluorescent probes with proteins: coumarin 153 and 1,8-ANS in complex with holo- and apomyoglobin.荧光探针与蛋白质相互作用的表征：香豆素153和1,8-ANS与全肌红蛋白和脱辅基肌红蛋白复合物的相互作用
Photochem Photobiol. 2006 Nov-Dec;82(6):1586-90. doi: 10.1562/2006-07-06-RA-962.
8
Pressure-induced perturbation of ANS-apomyoglobin complex: frequency domain fluorescence studies on native and acidic compact states.压力诱导的自主神经系统 - 脱辅基肌红蛋白复合物的扰动：天然态和酸性致密态的频域荧光研究
Protein Sci. 1996 Jan;5(1):121-6. doi: 10.1002/pro.5560050115.
9
Dynamics of ANS binding to tuna apomyoglobin measured with fluorescence correlation spectroscopy.用荧光相关光谱法测量的ANS与金枪鱼脱辅基肌红蛋白结合的动力学。
Biophys J. 2001 Dec;81(6):3510-21. doi: 10.1016/S0006-3495(01)75982-6.
10
Resolution of tryptophan-ANS fluorescence energy transfer in apomyoglobin by site-directed mutagenesis.通过定点诱变解析脱辅基肌红蛋白中色氨酸-ANS荧光能量转移
Photochem Photobiol. 2002 Oct;76(4):381-4. doi: 10.1562/0031-8655(2002)076<0381:rotafe>2.0.co;2.
引用本文的文献
1
Origin of the polychromatic photoluminescence of zeolite confined Ag clusters: temperature- and co-cation-dependent luminescence.沸石限域银团簇多色光致发光的起源：温度和共阳离子依赖的发光
Chem Sci. 2022 Aug 24;13(39):11560-11569. doi: 10.1039/d2sc03197c. eCollection 2022 Oct 12.
2
Applications of phasors to in vitro time-resolved fluorescence measurements.相量在体外时间分辨荧光测量中的应用。
Anal Biochem. 2011 Mar 1;410(1):62-9. doi: 10.1016/j.ab.2010.11.010. Epub 2010 Nov 13.
3
Fluorescent N-arylaminonaphthalene sulfonate probes for amyloid aggregation of alpha-synuclein.用于α-突触核蛋白淀粉样聚集的荧光N-芳基萘磺酸盐探针。
Biophys J. 2008 Jun;94(12):4867-79. doi: 10.1529/biophysj.107.125211. Epub 2008 Mar 13.
4
Radiative decay engineering: biophysical and biomedical applications.辐射衰变工程：生物物理与生物医学应用
Anal Biochem. 2001 Nov 1;298(1):1-24. doi: 10.1006/abio.2001.5377.
5
On spectral relaxation in proteins.关于蛋白质中的光谱弛豫
Photochem Photobiol. 2000 Oct;72(4):421-37. doi: 10.1562/0031-8655(2000)072<0421:OSRIP>2.0.CO;2.
6
Probing pH and pressure effects on the apomyoglobin heme pocket with the 2'-(N,N-dimethylamino)-6-naphthoyl-4-trans-cyclohexanoic acid fluorophore.用2'-（N，N-二甲基氨基）-6-萘甲酰基-4-反式环己烷羧酸荧光团探究pH值和压力对脱辅基肌红蛋白血红素口袋的影响。
Biophys J. 1996 Jun;70(6):2903-14. doi: 10.1016/S0006-3495(96)79860-0.
7
Dynamics of interaction of RNA polymerase II with nucleosomes. I. Effect of salts.RNA聚合酶II与核小体相互作用的动力学。I. 盐的影响。
Protein Sci. 1993 Dec;2(12):2233-45. doi: 10.1002/pro.5560021223.
8
Dynamic properties of the lipid-water interface of model membranes as revealed by lifetime-resolved fluorescence emission spectra.通过寿命分辨荧光发射光谱揭示的模型膜脂-水界面的动态特性。
Biochemistry. 1981 Mar 3;20(5):1366-73. doi: 10.1021/bi00508a051.
9
Theory of phase-modulation fluorescence spectroscopy for excited-state processes.用于激发态过程的相位调制荧光光谱理论。
Biophys Chem. 1982 Oct;16(2):99-115. doi: 10.1016/0301-4622(82)85012-6.
10
Analysis of fluorescence decay kinetics from variable-frequency phase shift and modulation data.基于可变频率相移和调制数据的荧光衰减动力学分析。
Biophys J. 1984 Oct;46(4):463-77. doi: 10.1016/S0006-3495(84)84043-6.
本文引用的文献
1
The interaction of a naphthalene dye with apomyoglobin and apohemoglobin. A fluorescent probe of non-polar binding sites.萘染料与脱辅基肌红蛋白和脱辅基血红蛋白的相互作用。一种非极性结合位点的荧光探针。
J Mol Biol. 1965 Sep;13(2):482-95. doi: 10.1016/s0022-2836(65)80111-5.
2
Quantitative estimation of protein binding site polarity. Fluorescence of N-arylaminonaphthalenesulfonates.蛋白质结合位点极性的定量评估。N-芳基氨基萘磺酸盐的荧光
Biochemistry. 1968 Oct;7(10):3381-90. doi: 10.1021/bi00850a011.
3
Evidence for binding of rose bengal and anilinonaphthalenesulfonates at the active site regions of liver alcohol dehydrogenase.孟加拉玫瑰红和苯胺基萘磺酸盐在肝脏乙醇脱氢酶活性位点区域结合的证据。
Biochemistry. 1967 Nov;6(11):3510-8. doi: 10.1021/bi00863a024.
4
Fluorescence studies of Aplysia and sperm whale apomyoglobins.海兔和抹香鲸脱辅基肌红蛋白的荧光研究。
Biochemistry. 1970 Nov 24;9(24):4723-9. doi: 10.1021/bi00826a015.
5
1-anilinonaphthalene-8-sulphonate. The dependence of emission spectra on molecular conformation studied by fluorescence and proton-magnetic resonance.
Eur J Biochem. 1972 Feb 15;25(2):218-28. doi: 10.1111/j.1432-1033.1972.tb01687.x.
6
On the analysis of fluorescence decay kinetics by the method of least-squares.通过最小二乘法对荧光衰减动力学进行分析。
Anal Biochem. 1974 Jun;59(2):583-98. doi: 10.1016/0003-2697(74)90312-1.
7
Fluorescence probes for structure.用于结构的荧光探针。
Annu Rev Biochem. 1972;41:843-68. doi: 10.1146/annurev.bi.41.070172.004211.
8
Fluorescent probes for conformational states of proteins. I. Mechanism of fluorescence of 2-p-toluidinylnaphthalene-6-sulfonate, a hydrophobic probe.用于蛋白质构象状态的荧光探针。I. 疏水探针2-对甲苯胺基萘-6-磺酸盐的荧光机制。
Biochemistry. 1966 Jun;5(6):1908-19. doi: 10.1021/bi00870a018.
9
Analysis of fluorescence decay curves by means of the Laplace transformation.通过拉普拉斯变换分析荧光衰减曲线。
Biophys J. 1975 Mar;15(3):263-80. doi: 10.1016/S0006-3495(75)85817-6.
10
Neutral and Cationic Sulfonamido Derivatives of the Fluorescent Probe 2-p-Toluidinylnaphthalene-6-sulfonate. Properties and Mechanistic implications.荧光探针2-对甲苯胺基萘-6-磺酸盐的中性和阳离子磺酰胺衍生物。性质及机理解析
Biochemistry. 1975 Feb 25;14(4):747-53. doi: 10.1021/bi00675a016.
Zotero 插件