吸附于L-α-卵磷脂囊泡的荧光探针的纳秒时间分辨发射光谱。
Nanosecond time-resolved emission spectroscopy of a fluorescence probe adsorbed to L-alpha-egg lecithin vesicles.
作者信息
Easter J H, DeToma R P, Brand L
出版信息
Biophys J. 1976 Jun;16(6):571-83. doi: 10.1016/S0006-3495(76)85712-8.
Abstract
Nanosecond time-resolved emission spectra (TRES) are fluorescence emission spectra obtained at discrete times during the fluorescence decay. The complete data-set obtainable is a surface representing the intensity at all wavelengths and times during the emission decay time. When 2-p-toluidinonaphthalene-6-sulfonate (2,6 p-TNS) is adsorbed to egg lecithin vesicles, an excited-state reaction associated with energetic changes of the emitting species occurs on the nanosecond time scale. Convolution of the fluorescence decay with the excitation response introduces an artifact in the time-dependent spectra. A precedure is described by which this artifact can be eliminated. The data for the generation of time-resolved emission spectra are obtained with a computer-interfaced instrument based on the single-photon counting method.
摘要
纳秒时间分辨发射光谱（TRES）是在荧光衰减过程中的离散时间获得的荧光发射光谱。可获得的完整数据集是一个表示发射衰减时间内所有波长和时间处强度的曲面。当2-对甲苯胺基萘-6-磺酸盐（2,6 p-TNS）吸附到卵磷脂囊泡上时，在纳秒时间尺度上会发生与发射物种能量变化相关的激发态反应。荧光衰减与激发响应的卷积在时间相关光谱中引入了伪像。本文描述了一种可以消除这种伪像的方法。用于生成时间分辨发射光谱的数据是通过基于单光子计数法的计算机接口仪器获得的。
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