Fenwick L, Jones R L, Naylor B, Poyser N L, Wilson N H
Br J Pharmacol. 1977 Jan;59(1):191-9. doi: 10.1111/j.1476-5381.1977.tb06993.x.
1 Prostaglandin production by rat uterus homogenates has been studied, in vitro, on days 2 to 13 of pseudopregnancy. 2 The highest production of prostaglandins occurred on day 5. 3 The amounts of prostaglandins F and D formed were higher than the amounts of prostaglandin E on every day studied. 4 The ratios of prostglandins F and D to prostaglandin E produced steadily decreased up to day 6. It then increased with the highest values occurring between days 10 and 13, 5 Progesterone levels in peripheral plasma increased rapidly from days 2 to 5, remained high up to day 9, then steadily decreased between days 10 and 13. 6 The anti-oestrogenic drug, tamoxifen administered on day 2, significantly inhibited the increase of prostaglandin production which occurred on day 5. Prostaglandin E production was inhibited more than the production of prostaglandins F and D. 7 Analysis of the uterine extracts by gas chromatography and mass spectrometry showed prostaglandin F2alpha, F1alpha (in trace amounts), E2 and D2 to be present. 8 The major product detected was 6-keto-prostaglandin F1alpha. Its identification forms an addendum to the paper. 9 Also present as a major product was 6(9)-oxy-11,15-dihydroxyprosta-7,13-dienoic acid.
已在体外研究了假孕第2至13天大鼠子宫匀浆中前列腺素的产生情况。
前列腺素的最高产量出现在第5天。
在研究的每一天，所形成的前列腺素F和D的量都高于前列腺素E的量。
直至第6天，前列腺素F和D与所产生的前列腺素E的比率稳步下降。然后该比率上升，在第10至13天达到最高值。
外周血浆中的孕酮水平从第2天到第5天迅速升高，直至第9天一直保持在高位，然后在第10至13天稳步下降。
在第2天给予抗雌激素药物他莫昔芬，可显著抑制第5天出现的前列腺素产量增加。前列腺素E的产量比前列腺素F和D的产量受到的抑制更大。
通过气相色谱法和质谱法对子宫提取物进行分析，结果显示存在前列腺素F2α、F1α（微量）、E2和D2。
检测到的主要产物是6-酮-前列腺素F1α。其鉴定结果作为本文的附录。
同样作为主要产物存在的是6(9)-氧代-11,15-二羟基前列腺-7,13-二烯酸。
