Takagi T, Yamamoto T, Asano S, Tamaki H
Biological Laboratory, Tanabe Seiyaku Co., Saitama, Japan.
Calcif Tissue Int. 1993 Jun;52(6):442-6. doi: 10.1007/BF00571334.
We studied the effects of prostaglandin D2 (PGD2) on the femoral bone mineral density (BMD) and other related parameters in ovariectomized (OVx) and sham-operated rats. BMD was measured in vivo by dual energy X-ray absorptiometry (DXA) for the period of 36 days or 112 days after operation. When 9- or 10-week-old rats were used at the time of operation, the femoral BMD increased during these periods. Ovariectomy resulted in a marked suppression of this steady increase in BMD at both proximal and distal ends of the femur. Subcutaneous administration of a slow-release preparation of PGD2 on days 1 and 21 not only prevented the ovariectomy-induced suppression of BMD, but also augmented the steady increase in BMD of the sham-operated rats. When medication was started on day 70, the depressed rate of increase in BMD was restored to the control level. Serum calcitonin (CT) and parathyroid hormone (PTH) levels were not affected by either ovariectomy or by PGD2 administration. Body weight and bone length were increased, but uterine weight was decreased by ovariectomy. PGD2 administration showed no effects on these parameters. There was a significant increase in the fasting level of urinary hydroxyproline excretion after ovariectomy, and PGD2 administration had no significant effect on this parameter either. These results indicate that the prevention of osteopenia in OVx rats and the increase in BMD in sham-operated and post-OVx rats by PGD2 administration are due to its stimulatory effect on bone formation.
我们研究了前列腺素D2（PGD2）对去卵巢（OVx）大鼠和假手术大鼠股骨骨密度（BMD）及其他相关参数的影响。术后36天或112天，通过双能X线吸收法（DXA）在体内测量骨密度。当手术时使用9或10周龄的大鼠时，在此期间股骨骨密度增加。去卵巢导致股骨近端和远端骨密度的这种稳定增加受到明显抑制。在第1天和第21天皮下注射PGD2缓释制剂，不仅可防止去卵巢引起的骨密度抑制，还可增强假手术大鼠骨密度的稳定增加。当在第70天开始用药时，骨密度降低的增加率恢复到对照水平。血清降钙素（CT）和甲状旁腺激素（PTH）水平不受去卵巢或PGD2给药的影响。去卵巢可使体重和骨长度增加，但子宫重量减少。PGD2给药对这些参数无影响。去卵巢后尿羟脯氨酸排泄的空腹水平显著增加，PGD2给药对该参数也无显著影响。这些结果表明，PGD2给药预防OVx大鼠骨质减少以及增加假手术和OVx后大鼠的骨密度是由于其对骨形成的刺激作用。
