Thurnher M, Clausen H, Sharon N, Berger E G
Institute of Physiology, University of Zurich, Switzerland.
Immunol Lett. 1993 Jun;36(3):239-43. doi: 10.1016/0165-2478(93)90095-j.
The Tn antigen (GalNAc alpha 1-O-Ser/Thr) is a disease-related O-linked (mucin-type) carbohydrate neoantigen which is expressed in idiopathic Tn syndrome, AIDS, T-cell lymphoma and in many carcinomas. In the present study, we took advantage of a Tn antigen expressing T-lymphocyte clone derived from a patient with the idiopathic form of the Tn syndrome and the Tn+ Jurkat cell line to characterize new reagents that should identify Tn antigens (monoclonal antibody 5F4 and a lectin newly isolated from Moluccella laevis seeds). Flow cytometry revealed that both reagents strongly bound to Tn antigen expressing T lymphocytes but not to normal donor T cells, which are Tn negative. In contrast to mAb 5F4, Moluccella laevis lectin weakly bound to normal donor cells after sialidase pretreatment, indicating its broader specificity. N-Acetyl-D-galactosamine at a concentration of 100 mM significantly reduced antibody binding and abolished lectin binding, completely demonstrating the sugar specificity of both reagents. These reagents should be useful tools in glycobiology and for clinical purposes.
Tn抗原（N-乙酰半乳糖胺α1-O-丝氨酸/苏氨酸）是一种与疾病相关的O-连接（粘蛋白型）碳水化合物新抗原，在特发性Tn综合征、艾滋病、T细胞淋巴瘤及多种癌组织中表达。在本研究中，我们利用从一名特发性Tn综合征患者获得的表达Tn抗原的T淋巴细胞克隆以及Tn+ Jurkat细胞系，对可识别Tn抗原的新型试剂（单克隆抗体5F4和一种从皱叶锦葵种子中新分离的凝集素）进行特性分析。流式细胞术显示，这两种试剂均能与表达Tn抗原的T淋巴细胞强烈结合，但不与Tn阴性的正常供体T细胞结合。与单克隆抗体5F4不同，经唾液酸酶预处理后，皱叶锦葵凝集素与正常供体细胞的结合较弱，表明其具有更广泛的特异性。浓度为100 mM的N-乙酰-D-半乳糖胺可显著降低抗体结合，并消除凝集素结合，充分证明了这两种试剂的糖特异性。这些试剂在糖生物学及临床应用中应是有用的工具。
