用于流行病学研究的空肠弯曲菌DNA的脉冲场凝胶电泳分析。
Pulsed-field gel electrophoretic analysis of Campylobacter jejuni DNA for use in epidemiological studies.
作者信息
Suzuki Y, Ishihara M, Funabashi M, Suzuki R, Isomura S, Yokochi T
机构信息
Laboratory of Bacteriology, Aichi Prefectural Institute of Public Health, Japan.
出版信息
J Infect. 1993 Jul;27(1):39-42. doi: 10.1016/0163-4453(93)93628-h.
The cleavage patterns of the genomic DNA of 42 clinical isolates of Campylobacter jejuni determined by pulsed-field gel electrophoresis (PFGE) were compared with their Lior and TCK serotypes. The fragment patterns of DNA obtained with SalI and SmaI restriction enzymes did not always accord with the corresponding serotypes but strains of the same serotype could be further divided into subtypes by their cleavage patterns. This PFGE method may prove useful for subclassifying C. jejuni.
通过脉冲场凝胶电泳（PFGE）测定的42株空肠弯曲菌临床分离株的基因组DNA切割模式与其Lior和TCK血清型进行了比较。用SalI和SmaI限制性内切酶获得的DNA片段模式并不总是与相应的血清型一致，但相同血清型的菌株可根据其切割模式进一步分为亚型。这种PFGE方法可能对空肠弯曲菌的亚分类有用。
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